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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3 Pt 2
|
| pubmed:dateCreated |
1992-5-4
|
| pubmed:abstractText |
Immunodissected rabbit cortical collecting duct (CCD) cells were grown in primary culture on permeable membrane supports. Transepithelial voltage, Na+, K+, and H+ gradients developed as expected for a mixed population of principal and intercalated cells. The amiloride-sensitive short-circuit current (Isc) was measured in Ussing chambers as an index of Na+ transport via apical membrane Na+ channels. Treatment of the cells in culture with 10 nM aldosterone for 48 h increased Isc from 7.4 +/- 1.4 to 19.3 +/- 3.2 microA/cm2. In contrast to the native rabbit CCD, 220 pM arginine vasopressin (AVP) produced a rapid and stable (greater than 60 min) increase in Isc to 15.8 +/- 2.0 and 29.0 +/- 3.8 microA/cm2 in untreated and aldosterone-treated cultures, respectively. Although prostaglandin E2 (PGE2) inhibits Na+ transport in the native rabbit CCD, it did not in the cultured cells, and it has previously been shown that PGE2 inhibition of AVP-dependent adenosine 3',5'-cyclic monophosphate production is lost in culture (W. K. Sonnenburg and W. L. Smith, J. Biol. Chem. 263: 6155-6160, 1988). We conclude that the development of a stable stimulation of Na+ transport by AVP is linked to the loss of the inhibitory effects of PGE2.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aldosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Arginine Vasopressin,
http://linkedlifedata.com/resource/pubmed/chemical/Furosemide,
http://linkedlifedata.com/resource/pubmed/chemical/Ouabain,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
262
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
F454-61
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1558162-Aldosterone,
pubmed-meshheading:1558162-Amiloride,
pubmed-meshheading:1558162-Animals,
pubmed-meshheading:1558162-Antibodies, Monoclonal,
pubmed-meshheading:1558162-Arginine Vasopressin,
pubmed-meshheading:1558162-Biological Transport,
pubmed-meshheading:1558162-Cell Separation,
pubmed-meshheading:1558162-Cells, Cultured,
pubmed-meshheading:1558162-Epithelium,
pubmed-meshheading:1558162-Furosemide,
pubmed-meshheading:1558162-Hydrogen-Ion Concentration,
pubmed-meshheading:1558162-Kidney Cortex,
pubmed-meshheading:1558162-Kidney Tubules, Collecting,
pubmed-meshheading:1558162-Kinetics,
pubmed-meshheading:1558162-Ouabain,
pubmed-meshheading:1558162-Potassium,
pubmed-meshheading:1558162-Rabbits,
pubmed-meshheading:1558162-Sodium
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
AVP stimulates Na+ transport in primary cultures of rabbit cortical collecting duct cells.
|
| pubmed:affiliation |
Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|