Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-12-20
pubmed:databankReference
pubmed:abstractText
MD simulation of sensory rhodopsin II was executed for three intermediates (ground-state, K-state, M-state) appearing in its photocycle. We observed a large displacement of the cytoplasmic side of helixF only in M-state among the three intermediates. This displacement was transmitted to TM2, and the cytoplasmic side of TM2 rotated clockwise. These transient movements are in agreement with the results of an EPR experiment. That is, the early stage of signal transduction in a sRII-HtrII complex was successfully reproduced by the in silico MD simulation. By analyzing the structure of the sRII-HtrII complex, the following findings about the photocycle of sRII were obtained: (1) The hydrogen bonds between helixF and other helices determine the direction of the movement of helixF; (2) three amino acids (Arg162, Thr189, Tyr199) are essential for sRII-HtrII binding and contribute to the motion transfer from sRII to HtrII; (3) after the isomerization of retinal, a major conformational change of retinal was caused by proton transfer from Schiff base to Asp75, which, in turn, triggers the steric collision of retinal with Trp171. This is the main reason for the movement of the cytoplasmic side of helixF.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-10024456, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-10512834, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-10653781, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-10920028, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-10966793, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-11478891, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-11504917, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-11574462, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-11937052, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-12368857, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-12496114, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-12842471, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-12860139, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-14653584, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-14971958, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-2001671, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-2252905, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-7708770, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-8334149, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-8428572, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-9144172, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-9254694, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-9772173, http://linkedlifedata.com/resource/pubmed/commentcorrection/15576566-9927658
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0961-8368
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
183-92
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Computational analysis of the transient movement of helices in sensory rhodopsin II.
pubmed:affiliation
Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 263-8522, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't