Source:http://linkedlifedata.com/resource/pubmed/id/15576443
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2005-3-17
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| pubmed:abstractText |
Myogenic tone of small arteries is dependent on the presence of extracellular calcium (Ca(o)(2+)), and, recently, a receptor that senses changes in Ca(2+), the calcium-sensing receptor (CaR), has been detected in vascular tissue. We investigated whether the CaR is involved in the regulation of myogenic tone in rat subcutaneous small arteries. Immunoblot analysis using a monoclonal antibody against the CaR demonstrated its presence in rat subcutaneous arteries. To determine whether the CaR was functionally active, segments of artery (< 250 microm internal diameter) mounted in a pressure myograph with an intraluminal pressure of 70 mmHg were studied after the development of myogenic tone. Increasing Ca(o)(2+) concentration ([Ca(2+)](o)) cumulatively from 0.5 to 10 mM induced an initial constriction (0.5-2 mM) followed by dilation (42 +/- 5% loss of tone). The dose-dependent dilation was mimicked by other known CaR agonists including magnesium (1-10 mM) and the aminoglycosides neomycin (0.003-10 mM) and kanamycin (0.003-3 mM). PKC activation with the phorbol ester phorbol-12,13-dibutyrate (20nM) inhibited the dilation induced by high [Ca(2+)](o) or neomycin, whereas inhibition of PKC with GF109203X (10 microM) increased the responses to Ca(o)(2+) or neomycin, consistent with the role of PKC as a negative regulator of the CaR. We conclude that rat subcutaneous arteries express a functionally active CaR that may be involved in the modulation of myogenic tone and hence the regulation of peripheral vascular resistance.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aminoglycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Calcium-Sensing
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0363-6135
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
288
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
H1756-62
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15576443-Aminoglycosides,
pubmed-meshheading:15576443-Animals,
pubmed-meshheading:15576443-Antibodies, Monoclonal,
pubmed-meshheading:15576443-Arteries,
pubmed-meshheading:15576443-Calcium,
pubmed-meshheading:15576443-Calcium Signaling,
pubmed-meshheading:15576443-Immunohistochemistry,
pubmed-meshheading:15576443-MAP Kinase Signaling System,
pubmed-meshheading:15576443-Magnesium,
pubmed-meshheading:15576443-Muscle, Smooth, Vascular,
pubmed-meshheading:15576443-Muscle Contraction,
pubmed-meshheading:15576443-Protein Kinase C,
pubmed-meshheading:15576443-Rats,
pubmed-meshheading:15576443-Rats, Sprague-Dawley,
pubmed-meshheading:15576443-Receptors, Calcium-Sensing,
pubmed-meshheading:15576443-Subcutaneous Tissue,
pubmed-meshheading:15576443-Vascular Resistance,
pubmed-meshheading:15576443-Vasoconstriction
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| pubmed:year |
2005
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| pubmed:articleTitle |
Evidence for a functional calcium-sensing receptor that modulates myogenic tone in rat subcutaneous small arteries.
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| pubmed:affiliation |
University Dept. of Medicine, University of Manchester and Manchester Royal Infirmary, Oxford Road, Manchester M13 9WL, UK. johanian@man.ac.uk
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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