Source:http://linkedlifedata.com/resource/pubmed/id/15574737
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
48
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| pubmed:dateCreated |
2004-12-2
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| pubmed:abstractText |
Our previous studies found that serotonin transporter (SERT) knock-out mice showed increased sensitivity to minor stress and increased anxiety-like behavior but reduced locomotor activity. These mice also showed decreased density of 5-hydroxytryptamine (5-HT1A) receptors in the hypothalamus, amygdala, and dorsal raphe. To evaluate the contribution of hypothalamic 5-HT1A receptors to these phenotypes of SERT knock-out mice, two studies were conducted. Recombinant adenoviruses containing 5-HT1A sense and antisense sequences (Ad-1AP-sense and Ad-1AP-antisense) were used to manipulate 5-HT1A receptors in the hypothalamus. The expression of the 5-HT1A genes is controlled by the 5-HT1A promoter, so that they are only expressed in 5-HT1A receptor-containing cells. (1) Injection of Ad-1AP-sense into the hypothalamus of SERT knock-out mice restored 5-HT1A receptors in the medial hypothalamus; this effect was accompanied by elimination of the exaggerated adrenocorticotropin responses to a saline injection (minor stress) and reduced locomotor activity but not by a change in increased exploratory anxiety-like behavior. (2) To further confirm the observation in SERT-/- mice, Ad-1AP-antisense was injected into the hypothalamus of normal mice. The density and the function of 5-HT1A receptors in the medial hypothalamus were significantly reduced in Ad-1AP-antisense-treated mice. Compared with the control group (injected with Ad-track), Ad-1A-antisense-treated mice showed a significant reduction in locomotor activity, but again no changes in exploratory anxiety-like behaviors, tested by elevated plus-maze and open-field tests. Thus, the present results demonstrate that medial hypothalamic 5-HT1A receptors regulate stress responses and locomotor activity but may not regulate exploratory anxiety-like behaviors.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenocorticotropic Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Corticosterone,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oxytocin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Serotonin, 5-HT1A,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SLC6A4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Plasma Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Slc6a4 protein, mouse
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
1529-2401
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
1
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| pubmed:volume |
24
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
10868-77
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15574737-Adenoviridae,
pubmed-meshheading:15574737-Adrenocorticotropic Hormone,
pubmed-meshheading:15574737-Animals,
pubmed-meshheading:15574737-Anxiety,
pubmed-meshheading:15574737-Cell Line,
pubmed-meshheading:15574737-Corticosterone,
pubmed-meshheading:15574737-DNA, Antisense,
pubmed-meshheading:15574737-Exploratory Behavior,
pubmed-meshheading:15574737-Female,
pubmed-meshheading:15574737-Genetic Vectors,
pubmed-meshheading:15574737-Humans,
pubmed-meshheading:15574737-Hypothalamus, Middle,
pubmed-meshheading:15574737-Injections,
pubmed-meshheading:15574737-Kidney,
pubmed-meshheading:15574737-Locomotion,
pubmed-meshheading:15574737-Male,
pubmed-meshheading:15574737-Maze Learning,
pubmed-meshheading:15574737-Membrane Glycoproteins,
pubmed-meshheading:15574737-Membrane Transport Proteins,
pubmed-meshheading:15574737-Mice,
pubmed-meshheading:15574737-Mice, Inbred C57BL,
pubmed-meshheading:15574737-Mice, Knockout,
pubmed-meshheading:15574737-Nerve Tissue Proteins,
pubmed-meshheading:15574737-Oxytocin,
pubmed-meshheading:15574737-Promoter Regions, Genetic,
pubmed-meshheading:15574737-Receptor, Serotonin, 5-HT1A,
pubmed-meshheading:15574737-Recombinant Fusion Proteins,
pubmed-meshheading:15574737-Serotonin Plasma Membrane Transport Proteins,
pubmed-meshheading:15574737-Stress, Physiological
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| pubmed:year |
2004
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| pubmed:articleTitle |
Medial hypothalamic 5-hydroxytryptamine (5-HT)1A receptors regulate neuroendocrine responses to stress and exploratory locomotor activity: application of recombinant adenovirus containing 5-HT1A sequences.
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| pubmed:affiliation |
Department of Psychiatry and Behavioral Sciences, University of Texas Medical Branch, Galveston, Texas 77555-0431, USA. i@utmb.edu
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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