Linked Life Data

15567719

Source:http://linkedlifedata.com/resource/pubmed/id/15567719

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-11-29
pubmed:databankReference
pubmed:abstractText
In mouse male germ cells, global DNA methylation occurs in gonocytes at 16-18 days postcoitum. In the present study, we examined which de novo-type DNA methyltransferase, Dnmt3a, Dnmt3a2 or Dnmt3b is expressed in gonocytes at these stages. Immuno-histochemical and Western blot analyses revealed that Dnmt3a2 was the major DNA methyltransferase expressed in gonocytes at 14-18 day postcoitum. Dnmt3L, which is necessary for spermatogenesis, was co-expressed in gonocytes at identical stages to Dnmt3a2. On the other hand, Dnmt3a was expressed not in germ cells but in the Sertoli cells and connective tissue cells that surround gonocytes and spermatogonia. Dnmt3b2, an isoform of Dnmt3b, was expressed faintly but significantly in gonocytes at 16 days postcoitum, and increased in spermatogonia at 4 and 6 days postpartum. The expression of Dnmt3a2, Dnmt3L, and Dnmt3b2 at 14-18 dpc was confirmed by reverse transcriptase-coupled polymerase chain reaction amplification and nucleotide sequencing of the amplified fragments. The results strongly suggest that Dnmt3a2 and Dnmt3L are responsible for the global DNA methylation in mouse male germ cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1567-133X
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
231-7
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15567719-Amino Acid Sequence, pubmed-meshheading:15567719-Animals, pubmed-meshheading:15567719-Animals, Newborn, pubmed-meshheading:15567719-DNA (Cytosine-5-)-Methyltransferase, pubmed-meshheading:15567719-DNA Methylation, pubmed-meshheading:15567719-Embryo, Mammalian, pubmed-meshheading:15567719-Gene Expression Regulation, Developmental, pubmed-meshheading:15567719-Gene Expression Regulation, Enzymologic, pubmed-meshheading:15567719-Immunohistochemistry, pubmed-meshheading:15567719-Male, pubmed-meshheading:15567719-Mice, pubmed-meshheading:15567719-Molecular Sequence Data, pubmed-meshheading:15567719-Sertoli Cells, pubmed-meshheading:15567719-Spermatogenesis, pubmed-meshheading:15567719-Spermatogonia, pubmed-meshheading:15567719-Spermatozoa, pubmed-meshheading:15567719-Testis
pubmed:year
2004
pubmed:articleTitle
Co-expression of de novo DNA methyltransferases Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos.
pubmed:affiliation
Department of Anatomy, Kitasato University School of Medicine, Sagamihara 228-8555, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't