Source:http://linkedlifedata.com/resource/pubmed/id/15567233
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2004-11-29
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pubmed:abstractText |
Bacterial culture was allowed to dry to completeness on Columbia agar base with defibrinated horse blood. Following 6 months storage at room temperature, microbial DNA was extracted and successfully amplified by PCR. This storage technique has the advantage over other methods of not requiring (i) a DNA extraction protocol prior to storage and (ii) refrigeration and/or freezing. This technique maybe useful in the transportation of bacterial genomic DNA in nonviable cells as well as reliable method for the storage of DNA in underdeveloped countries.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0167-7012
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131-3
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15567233-Base Sequence,
pubmed-meshheading:15567233-DNA, Bacterial,
pubmed-meshheading:15567233-Enterobacter cloacae,
pubmed-meshheading:15567233-Enterococcus faecium,
pubmed-meshheading:15567233-Humans,
pubmed-meshheading:15567233-Molecular Sequence Data,
pubmed-meshheading:15567233-Polymerase Chain Reaction,
pubmed-meshheading:15567233-Preservation, Biological,
pubmed-meshheading:15567233-Pseudomonas aeruginosa,
pubmed-meshheading:15567233-Sequence Analysis, DNA
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pubmed:year |
2005
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pubmed:articleTitle |
A simple and economic preservation method for genomic bacterial DNA from clinically significant pathogens.
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pubmed:affiliation |
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Lisburn Road, Belfast BT9 7AD, Northern Ireland, United Kingdom. jemoore@niphl.dnet.co.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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