Source:http://linkedlifedata.com/resource/pubmed/id/15567038
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2004-11-29
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pubmed:abstractText |
A novel coronavirus (CoV) has been described in association with cases of severe acute respiratory syndrome (SARS). The virus, SARS-CoV, differs from the previously described human coronaviruses, 229E and OC43. 229E was previously shown to productively infect human monocytes/macrophages, whereas OC43 poorly infected the cells. In this study, we examined whether SARS-CoV could productively infect purified monocytes/macrophages (PM) derived from human donor cells. Unlike 229E-infected cells, which produced viral titers of 10(3.5) to 10(6)TCID50/ml, SARS-CoV replicated poorly in PM, producing titers of 10(1.75) to 10(2)TCID50/ml. This finding was similar to results reported for OC43-infected cells, with titers ranging from 10(1.2) to 10(2.7)TCID50/ml. Of interest, SARS-CoV proteins were detected only in PM that did not produce significant amounts of interferon (IFN)-alpha, and in one such case, preliminary electron microscope studies demonstrated that SARS-CoV-like particles could enter the cells, possibly via phagocytosis. These results suggest that SARS-CoV, like human CoV OC43, poorly infects human PM, and production of IFN-alpha by these cells further limits the infection. Given the importance of monocytes/macrophages to the immune response, it is possible that their infection by SARS-CoV and alteration of this infection by IFN-alpha may be important to the course of the infection in humans.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0168-1702
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
107
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-101
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15567038-Adult,
pubmed-meshheading:15567038-Antibodies, Viral,
pubmed-meshheading:15567038-Humans,
pubmed-meshheading:15567038-Interferon-alpha,
pubmed-meshheading:15567038-Macrophages,
pubmed-meshheading:15567038-Microscopy, Electron,
pubmed-meshheading:15567038-Monocytes,
pubmed-meshheading:15567038-Phagocytosis,
pubmed-meshheading:15567038-SARS Virus,
pubmed-meshheading:15567038-Severe Acute Respiratory Syndrome,
pubmed-meshheading:15567038-Virus Replication
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pubmed:year |
2005
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pubmed:articleTitle |
SARS-coronavirus replication in human peripheral monocytes/macrophages.
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pubmed:affiliation |
Respiratory and Enteric Viruses Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS-C22, Atlanta, GA 30333, USA. mby7@cdc.gov
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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