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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 25
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pubmed:dateCreated |
2004-11-26
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pubmed:abstractText |
Adipocyte differentiation is controlled by complex actions involving gene expression and signal transduction. From metaphase to anaphase, peroxisome proliferator-activated receptor gamma, the CCAAT/enhancer-binding protein family and sterol regulatory element-binding protein-1 are known to function as master regulators. However, the mechanism underlying the earliest step, which triggers the initiation of differentiation, remains unknown. In previous reports, we have isolated a number of genes, whose expression increases in the early stage of differentiation in the mouse 3T3-L1 preadipocyte cell line. Here we report the cloning of the full-length cDNA and characterization of an unknown gene isolated previously and named fad24 (factor for adipocyte differentiation 24). Fad24 encodes a protein consisting of 807 amino acids. The deduced amino acid sequence was shown to have a basic leucine zipper motif and a NOC domain. Expression of fad24 was rapidly induced after stimulation with inducers. Furthermore, overexpression of fad24 in NIH-3T3 cells promoted adipogenesis in the presence of a ligand for peroxisome proliferator-activated receptor gamma. FAD24 localizes in the nucleus, especially within nuclear speckles. As the nuclear speckle functions as a nascent transcription and pre-mRNA splicing machinery, there is a possibility that FAD24 functions as one of the components for transcription and/or pre-mRNA splicing and positively regulates adipocyte differentiation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Basic-Leucine Zipper Transcription...,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/NOC3 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleocytoplasmic Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PPAR gamma,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SREBF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Srebf1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Sterol Regulatory Element Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9533
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
117
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6217-26
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15564382-3T3-L1 Cells,
pubmed-meshheading:15564382-Adipocytes,
pubmed-meshheading:15564382-Amino Acid Sequence,
pubmed-meshheading:15564382-Anaphase,
pubmed-meshheading:15564382-Animals,
pubmed-meshheading:15564382-Basic-Leucine Zipper Transcription Factors,
pubmed-meshheading:15564382-Blotting, Northern,
pubmed-meshheading:15564382-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:15564382-Cell Differentiation,
pubmed-meshheading:15564382-Cell Nucleus,
pubmed-meshheading:15564382-Cloning, Molecular,
pubmed-meshheading:15564382-DNA, Complementary,
pubmed-meshheading:15564382-DNA-Binding Proteins,
pubmed-meshheading:15564382-Gene Expression Regulation,
pubmed-meshheading:15564382-Green Fluorescent Proteins,
pubmed-meshheading:15564382-Humans,
pubmed-meshheading:15564382-Ligands,
pubmed-meshheading:15564382-Metaphase,
pubmed-meshheading:15564382-Mice,
pubmed-meshheading:15564382-Mice, Inbred C57BL,
pubmed-meshheading:15564382-Molecular Sequence Data,
pubmed-meshheading:15564382-NIH 3T3 Cells,
pubmed-meshheading:15564382-Nuclear Proteins,
pubmed-meshheading:15564382-Nucleocytoplasmic Transport Proteins,
pubmed-meshheading:15564382-PPAR gamma,
pubmed-meshheading:15564382-Protein Structure, Tertiary,
pubmed-meshheading:15564382-RNA, Messenger,
pubmed-meshheading:15564382-RNA Interference,
pubmed-meshheading:15564382-RNA Splicing,
pubmed-meshheading:15564382-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15564382-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:15564382-Sequence Homology, Amino Acid,
pubmed-meshheading:15564382-Sterol Regulatory Element Binding Protein 1,
pubmed-meshheading:15564382-Subcellular Fractions,
pubmed-meshheading:15564382-Time Factors,
pubmed-meshheading:15564382-Transcription Factors
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pubmed:year |
2004
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pubmed:articleTitle |
Fad24, a mammalian homolog of Noc3p, is a positive regulator in adipocyte differentiation.
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pubmed:affiliation |
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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