Linked Life Data

15564292

Source:http://linkedlifedata.com/resource/pubmed/id/15564292

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-1-24
pubmed:abstractText
Prostaglandin E(2) exerts its biological activity through binding to its membrane receptors, E-prostanoid (EP) receptors. Our previous finding that lack of EP(1) receptor inhibits the early stages of colon carcinogenesis led us to investigate whether EP(1) receptor deficiency reduces colon cancer development induced by azoxymethane (AOM) using EP(1) receptor knockout mice. At 6 weeks of age 33 homozygous EP(1)-deficient (EP(1)(-/-)) mice and 28 wild-type (EP(1)(+/+)) mice were given i.p. AOM (10 mg/kg body wt) once a week for 6 weeks. At 56 weeks of age all animals were killed and intestinal tumors were examined. The results clearly indicated that lack of EP(1) receptor significantly reduced colon cancer incidence (27 versus 57%, P < 0.05) and multiplicity (0.30 versus 0.76, P < 0.05) as well as tumor volume (12.2 versus 75.6 mm(3), P < 0.05). In EP(1)(-/-) mice, silver stained nucleolar organization region protein count as cell proliferation marker was significantly reduced (1.35 versus 2.17, P < 0.001) and apoptosis was significantly increased (0.685 versus 0.077, P < 0.001) in colon tumors induced by AOM compared with those in EP(1)(+/+) mice. We confirmed that EP(1) receptor mRNA was overexpressed in colon cancers of EP(1)(+/+) mice using reverse transcription-polymerase chain reaction. These results provide strong evidence that the EP(1) receptor is of major importance for colon cancer development and it could be a new target for a mechanism-based chemoprevention strategy against colon cancer development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
353-7
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Prostaglandin E receptor subtype EP(1) deficiency inhibits colon cancer development.
pubmed:affiliation
Cancer Prevention Basic Research Project, National Cancer Center Research Institute, 1-1 Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan. kawamori@musc.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't