Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2004-11-23
pubmed:abstractText
Terrein is a bioactive fungal metabolite whose effects are almost unknown. In this study, we found for the first time that terrein has a strong hypopigmentary effect in a spontaneously immortalized mouse melanocyte cell line, Mel-Ab. Treatment of Mel-Ab cells with terrein (10-100 microM) for 4 days significantly reduced melanin levels in a dose-dependent manner. In addition, terrein at the same concentration also reduced tyrosinase activity. We then investigated whether terrein influences the extracellular signal-regulated protein kinase (ERK) pathway and the expression of microphthalmia-associated transcription factor (MITF), which is required for tyrosinase expression. Terrein was found to induce sustained ERK activation and MITF down-regulation, and luciferase assays showed that terrein inhibits MITF promoter activity in a dose-dependent manner. To elucidate the correlation between ERK pathway activation and a decreased MITF transcriptional level, PD98059, a specific inhibitor of the ERK pathway, was applied before terrein treatment and found to abrogate the terrein-induced MITF attenuation. Terrein also reduced the tyrosinase protein level for at least 72 h. These results suggest that terrein reduces melanin synthesis by reducing tyrosinase production via ERK activation, and that this is followed by MITF down-regulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1420-682X
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2878-85
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15558216-Animals, pubmed-meshheading:15558216-Blotting, Western, pubmed-meshheading:15558216-Cell Line, pubmed-meshheading:15558216-Cell Line, Tumor, pubmed-meshheading:15558216-Cell Survival, pubmed-meshheading:15558216-Cyclopentanes, pubmed-meshheading:15558216-DNA-Binding Proteins, pubmed-meshheading:15558216-Dose-Response Relationship, Drug, pubmed-meshheading:15558216-Extracellular Signal-Regulated MAP Kinases, pubmed-meshheading:15558216-Luciferases, pubmed-meshheading:15558216-Melanins, pubmed-meshheading:15558216-Melanoma, Experimental, pubmed-meshheading:15558216-Mice, pubmed-meshheading:15558216-Microphthalmia-Associated Transcription Factor, pubmed-meshheading:15558216-Monophenol Monooxygenase, pubmed-meshheading:15558216-Penicillium, pubmed-meshheading:15558216-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
Terrein: a new melanogenesis inhibitor and its mechanism.
pubmed:affiliation
Department of Dermatology, Bundang Hospital, Seoul National University, 300 Gumi-Dong, Bundang-Gu, 463-707, Seongnam-Si, Kyoungki-Do, Korea.
pubmed:publicationType
Journal Article