rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
46
|
pubmed:dateCreated |
2004-11-19
|
pubmed:abstractText |
Single-chain and TRIS-assembled collagen mimetic peptide structures incorporating catechol groups were synthesized. When 1/3 equiv of Fe3+ was added to the single-chain compound in 50 mM CAPS buffer (pH 10), the 1:3 Fe3+-catechol complex that formed acted as an N-terminal scaffold to assemble the triple helix. When 1 equiv of Fe3+ was added to the TRIS-assembled compound in the buffer solution, the Fe3+-catechol complex acted as an extra C-terminal scaffold, which lead to a triple helix with both termini tethered. The formation of this C-terminal complex increased the Tm by a remarkable 22 degrees C!
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0002-7863
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
24
|
pubmed:volume |
126
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
15030-1
|
pubmed:dateRevised |
2008-1-17
|
pubmed:meshHeading |
pubmed-meshheading:15547991-Catechols,
pubmed-meshheading:15547991-Circular Dichroism,
pubmed-meshheading:15547991-Collagen,
pubmed-meshheading:15547991-Ferric Compounds,
pubmed-meshheading:15547991-Iron Chelating Agents,
pubmed-meshheading:15547991-Peptides,
pubmed-meshheading:15547991-Protein Structure, Quaternary,
pubmed-meshheading:15547991-Protein Structure, Secondary,
pubmed-meshheading:15547991-Spectrophotometry, Ultraviolet,
pubmed-meshheading:15547991-Tromethamine
|
pubmed:year |
2004
|
pubmed:articleTitle |
Metal-assisted assembly and stabilization of collagen-like triple helices.
|
pubmed:affiliation |
Department of Chemistry and Biochemistry, University of California-San Diego, La Jolla, California 92093, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|