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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-11-19
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pubmed:abstractText |
The NH2-terminal Jun kinases (JNKs) function in diverse roles through phosphorylation and activation of AP-1 components including ATF2 and c-Jun. However, the genes that mediate these processes are poorly understood. A model phenotype characterized by rapid activation of Jun kinase and enhanced DNA repair following cisplatin treatment was examined using chromatin immunoprecipitation with antibodies against ATF2 and c-Jun or their phosphorylated forms and hybridization to promoter arrays. Following genotoxic stress, we identified 269 genes whose promoters are bound upon phosphorylation of ATF2 and c-Jun. Binding did not occur following treatment with transplatin or the JNK inhibitor SP600125 or JNK-specific siRNA. Of 89 known DNA repair genes represented on the array, 23 are specifically activated by cisplatin treatment within 3-6 hr. Thus, the genotoxic stress response occurs at least partly via activation of ATF2 and c-Jun, leading to large-scale coordinate gene expression dominated by genes of DNA repair.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATF2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Anthracenes,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cisplatin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/anthra(1,9-cd)pyrazol-6(2H)-one
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
521-35
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15546613-Activating Transcription Factor 2,
pubmed-meshheading:15546613-Anthracenes,
pubmed-meshheading:15546613-Antineoplastic Agents,
pubmed-meshheading:15546613-Blotting, Western,
pubmed-meshheading:15546613-Cell Line, Tumor,
pubmed-meshheading:15546613-Chromatin Immunoprecipitation,
pubmed-meshheading:15546613-Cisplatin,
pubmed-meshheading:15546613-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:15546613-DNA Repair,
pubmed-meshheading:15546613-DNA-Binding Proteins,
pubmed-meshheading:15546613-Enzyme Inhibitors,
pubmed-meshheading:15546613-Gene Expression Profiling,
pubmed-meshheading:15546613-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:15546613-Humans,
pubmed-meshheading:15546613-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15546613-Phosphorylation,
pubmed-meshheading:15546613-Promoter Regions, Genetic,
pubmed-meshheading:15546613-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:15546613-RNA, Small Interfering,
pubmed-meshheading:15546613-Time Factors,
pubmed-meshheading:15546613-Transcription, Genetic,
pubmed-meshheading:15546613-Transcription Factors,
pubmed-meshheading:15546613-Transcriptional Activation
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pubmed:year |
2004
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pubmed:articleTitle |
Identification of promoters bound by c-Jun/ATF2 during rapid large-scale gene activation following genotoxic stress.
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pubmed:affiliation |
Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, California 92121, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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