Source:http://linkedlifedata.com/resource/pubmed/id/15543932
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
|
pubmed:dateCreated |
2004-11-16
|
pubmed:abstractText |
Iron may populate distinct hepatocellular iron pools that differentially regulate expression of proteins such as ferritin and transferrin receptor (TfR) through iron-regulatory mRNA-binding proteins (IRPs), and may additionally regulate uptake and accumulation of non-transferrin-bound iron (NTBI). We examined iron-regulatory protein (IRP) binding activity and ferritin/TfR expression in human hepatoma (HepG2) cells exposed to iron at different levels for different periods. Several iron-dependent RNA-binding activities were identified, but only IRP increased with beta-mercaptoethanol. With exposures between 0 and 20 microg/ml iron, decreases in IRP binding accompanied large changes in TfR and ferritin expression, while chelation of residual iron with deferoxamine (DFO) caused a large increase in IRP binding with little additional effect on TfR or ferritin expression. Cellular iron content increased beyond 4 days of exposure to iron at 20 microg/ml, when IRP binding, TfR, and ferritin had all reached stable levels. However, iron content of the cells plateaued by 7 days, or decreased with 24 h exposure to very high concentrations (>50 microg/ml) of iron. These results indicate that iron-replete HepG2 cells exhibit a narrow range of maximal responsiveness of the IRP-regulatory mechanism, whose functional response is blunted both by excessive iron exposure and by removal of iron from a chelatable pool. HepG2 cells are able to limit iron accumulation upon higher or prolonged exposure to NTBI, apparently independent of the IRP mechanism.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Edetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Ferritins,
http://linkedlifedata.com/resource/pubmed/chemical/Iron,
http://linkedlifedata.com/resource/pubmed/chemical/Iron-Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transferrin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
|
pubmed:issn |
0300-8177
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
265
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
37-45
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:15543932-Blotting, Western,
pubmed-meshheading:15543932-Cell Line,
pubmed-meshheading:15543932-Cell Line, Tumor,
pubmed-meshheading:15543932-Cell Proliferation,
pubmed-meshheading:15543932-Cytoplasm,
pubmed-meshheading:15543932-Dose-Response Relationship, Drug,
pubmed-meshheading:15543932-Edetic Acid,
pubmed-meshheading:15543932-Ferritins,
pubmed-meshheading:15543932-Hepatocytes,
pubmed-meshheading:15543932-Humans,
pubmed-meshheading:15543932-Iron,
pubmed-meshheading:15543932-Iron-Regulatory Proteins,
pubmed-meshheading:15543932-RNA,
pubmed-meshheading:15543932-Receptors, Transferrin,
pubmed-meshheading:15543932-Recombinant Proteins,
pubmed-meshheading:15543932-Time Factors
|
pubmed:year |
2004
|
pubmed:articleTitle |
Iron accumulation and iron-regulatory protein activity in human hepatoma (HepG2) cells.
|
pubmed:affiliation |
Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Circle, Toronto, Ont., Canada.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|