Linked Life Data

15541921

Source:http://linkedlifedata.com/resource/pubmed/id/15541921

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2004-11-15
pubmed:abstractText
The purpose of this study is to delineate uptake characteristics of mannosylated and fucosylated proteins in primary cultured sinusoidal endothelial cells and Kupffer cells. In cultured sinusoidal endothelial cells, uptake of mannosylated and fucosylated bovine serum albumin (BSA) was significantly inhibited by excess mannosylated and fucosylated BSAs but not by galactosylated BSA, suggesting that both glycosylated proteins might be primarily taken up via mannose receptors. In cultured Kupffer cells, uptake of fucosylated BSA was significantly inhibited by excess galactosylated BSA as well as mannosylated and fucosylated BSAs, although that of mannosylated BSA was inhibited only by mannosylated and fucosylated BSAs. This suggests that uptake of fucosylated BSA by Kupffer cells might be mediated by both Kupffer cell lectin (fucose receptor) and mannose receptor. On the other hand, in vivo hepatic uptake of fucosylated BSA was inhibited to a greater extent by GdCl3 pretreatment than that of mannosylated BSA. Based on in vitro and in vivo experiments, it was concluded that fucosylated BSA is more Kupffer cell-selective because it exhibited a lower sinusoidal endothelial cell uptake than mannosylated BSA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0378-5173
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
287
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
147-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Uptake characteristics of mannosylated and fucosylated bovine serum albumin in primary cultured rat sinusoidal endothelial cells and Kupffer cells.
pubmed:affiliation
Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606 8501, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't