Source:http://linkedlifedata.com/resource/pubmed/id/15541800
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2004-11-15
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| pubmed:abstractText |
The extended single-reaction multiplex PCR (esr-mPCR) developed in this study to detect staphylococcal enterotoxins (SEs), including SEA, SEB, SEC, SED, SEE, SEH, SEI, and SEJ, requires fewer sets of primers than other conventional multiplex PCRs and can be used to detect newly identified staphylococcal enterotoxins SEs more readily. Esr-mPCR analysis of 141 isolates of Staphylococcus aureus obtained from abattoir and livestock product samples revealed that 27 of the S. aureus isolates were toxigenic, and two were 2 multitoxigenic isolates. The most prevalent SE type was SEI followed by SEA and SEH. In addition, we investigated the clonal relatedness of toxigenic S. aureus isolates by arbitrarily primed PCR (AP-PCR). AP-PCR analysis of toxigenic S. aureus isolates revealed that the discriminatory power of AP-PCR was 9 (D=0.81), 8 (D=0.77), and 10 types (D=0.83) with primers AP1, ERIC2, and AP7, respectively. The combination of three each AP-PCR result could rearrange toxigenic S. aureus isolates into 10 types and five subtypes, with the D-value of 0.92. Interestingly, our data showed that toxigenic S. aureus isolates from different sources had different fingerprinting patterns although some of them carried the same types of SE genes. These data suggest that combinations of esr-mPCR and AP-PCR can provide a powerful approach for epidemiological investigation of toxigenic S. aureus isolates.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
|
| pubmed:issn |
0168-1605
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| pubmed:author |
pubmed-author:BaeW KWK,
pubmed-author:BohachG AGA,
pubmed-author:CHUS USU,
pubmed-author:FRYL RLR,
pubmed-author:JungW KWK,
pubmed-author:KimJ YJY,
pubmed-author:KwonN HNH,
pubmed-author:LEEF MFM,
pubmed-author:Los'V NVN,
pubmed-author:MOER ERE,
pubmed-author:MuraBB,
pubmed-author:ParkY HYH,
pubmed-author:ROYM YMY
|
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
97
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
137-45
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:15541800-Abattoirs,
pubmed-meshheading:15541800-Bacterial Typing Techniques,
pubmed-meshheading:15541800-DNA, Bacterial,
pubmed-meshheading:15541800-DNA Fingerprinting,
pubmed-meshheading:15541800-DNA Primers,
pubmed-meshheading:15541800-Enterotoxins,
pubmed-meshheading:15541800-Food Microbiology,
pubmed-meshheading:15541800-Polymerase Chain Reaction,
pubmed-meshheading:15541800-Staphylococcal Food Poisoning,
pubmed-meshheading:15541800-Staphylococcus aureus
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| pubmed:year |
2004
|
| pubmed:articleTitle |
Application of extended single-reaction multiplex polymerase chain reaction for toxin typing of Staphylococcus aureus isolates in South Korea.
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| pubmed:affiliation |
Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Shilim 9-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|