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pubmed-article:15541281pubmed:dateCreated2004-11-15lld:pubmed
pubmed-article:15541281pubmed:abstractTextExploitation of recombinant technology to study proteins containing strings of short consensus repeat (SCR) domains largely depends on expression vectors. In this paper, we describe a vector for cloning and constitutive expression of single or multiple SCR domains. The recombinant vector has unique additive features over commercially available vectors that make it a universal cloning vector for SCR domains as well as a vector suitable for expressing any protein fragment beginning and ending with cysteine residues. As a demonstration of its usefulness, the constitutive extracellular expression of five SCR-containing proteins derived from complement factor H is presented.lld:pubmed
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pubmed-article:15541281pubmed:issn0022-1759lld:pubmed
pubmed-article:15541281pubmed:authorpubmed-author:AlamM NurulMNlld:pubmed
pubmed-article:15541281pubmed:authorpubmed-author:PangburnMicha...lld:pubmed
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pubmed-article:15541281pubmed:authorpubmed-author:SreedharMalad...lld:pubmed
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pubmed-article:15541281pubmed:volume293lld:pubmed
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pubmed-article:15541281pubmed:pagination107-13lld:pubmed
pubmed-article:15541281pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:15541281pubmed:year2004lld:pubmed
pubmed-article:15541281pubmed:articleTitleA novel vector for the expression of SCR domains in insect cells.lld:pubmed
pubmed-article:15541281pubmed:affiliationDepartment of Biochemistry, University of Texas Health Science Center, 11937 U.S. Hwy 271, Tyler, TX 75703, USA.lld:pubmed
pubmed-article:15541281pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15541281pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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