Source:http://linkedlifedata.com/resource/pubmed/id/15541281
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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0021585,
umls-concept:C0086022,
umls-concept:C0185117,
umls-concept:C0205314,
umls-concept:C0442335,
umls-concept:C0679622,
umls-concept:C1514562,
umls-concept:C1539487,
umls-concept:C1705099,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2911684
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pubmed:issue |
1-2
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pubmed:dateCreated |
2004-11-15
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pubmed:abstractText |
Exploitation of recombinant technology to study proteins containing strings of short consensus repeat (SCR) domains largely depends on expression vectors. In this paper, we describe a vector for cloning and constitutive expression of single or multiple SCR domains. The recombinant vector has unique additive features over commercially available vectors that make it a universal cloning vector for SCR domains as well as a vector suitable for expressing any protein fragment beginning and ending with cysteine residues. As a demonstration of its usefulness, the constitutive extracellular expression of five SCR-containing proteins derived from complement factor H is presented.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1759
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
293
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
107-13
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15541281-Animals,
pubmed-meshheading:15541281-Cloning, Molecular,
pubmed-meshheading:15541281-Complement Factor H,
pubmed-meshheading:15541281-Genetic Vectors,
pubmed-meshheading:15541281-Insects,
pubmed-meshheading:15541281-Plasmids,
pubmed-meshheading:15541281-Protein Structure, Tertiary,
pubmed-meshheading:15541281-Transfection
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pubmed:year |
2004
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pubmed:articleTitle |
A novel vector for the expression of SCR domains in insect cells.
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pubmed:affiliation |
Department of Biochemistry, University of Texas Health Science Center, 11937 U.S. Hwy 271, Tyler, TX 75703, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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