Linked Life Data

15536001

Source:http://linkedlifedata.com/resource/pubmed/id/15536001

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
2004-11-10
pubmed:abstractText
Random mutagenesis combined with phenotypic screening using carefully crafted functional tests has successfully led to the discovery of genes that are essential for a number of functions. This approach does not require prior knowledge of the identity of the genes that are involved and is a way to ascribe function to the nearly 6000 genes for which knowledge of the DNA sequence has been inadequate to determine the function of the gene product. In an effort to identify genes involved in the visual system via this approach, we have tested over 9000 first and third generation offspring of mice treated with the mutagen N-ethyl-N-nitrosourea (ENU) for visual defects, as evidenced by abnormalities in the electroretinogram and appearance of the fundus. We identified 61 putative mutations with this procedure and outline the steps needed to identify the affected genes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0042-6989
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3335-45
pubmed:dateRevised
2011-6-6
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Results from screening over 9000 mutation-bearing mice for defects in the electroretinogram and appearance of the fundus.
pubmed:affiliation
Department of Neurobiology and Physiology and Center for Functional Genomics, Northwestern University, 2205 Tech Drive, Hogan Hall 2-140, Evanston, IL 60208, USA. larry-pinto@northwestern.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.