Source:http://linkedlifedata.com/resource/pubmed/id/15530414
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2004-11-8
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pubmed:abstractText |
Mesenchymal stem cells (MSCs) are able to differentiate into many types of cells including chondrocytes. Transforming growth factor beta1 (TGF-beta1) is very important in the regulation of chondrogenesis. Since cartilage-derived morphogenetic protein-1 (CDMP-1) belongs to the TGF-beta superfamily, we tested whether CDMP-1 plays any role in the regulation of the differentiation of MSCs into chondrocytes using a high density pellet culture system. Based on the histological staining of glycosaminoglycan using toluidine blue dye-binding method we found that CDMP-1 could initiate chondrogenic differentiation of MSCs as did TGF-beta1. However, CDMP-1 was less stimulatory than TGF-beta1. The combination of CDMP-1 and TGF-beta1 synergically induced chondrogenesis of MSCs. This synergic chondrogenic effect of CDMP-1 together with TGF-beta1 was further confirmed by quantification of GAG using dimethylmethylene blue dye-binding assay and immunohistochemical analysis of the expression of cartilage-specific protein collagen II. This study may provide an improved induction approach using MSCs for repairing damaged cartilage.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosaminoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Differentiation Factor 5,
http://linkedlifedata.com/resource/pubmed/chemical/TGFB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
325
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
453-60
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15530414-Aborted Fetus,
pubmed-meshheading:15530414-Bone Morphogenetic Proteins,
pubmed-meshheading:15530414-Cell Differentiation,
pubmed-meshheading:15530414-Cell Proliferation,
pubmed-meshheading:15530414-Chondrocytes,
pubmed-meshheading:15530414-Chondrogenesis,
pubmed-meshheading:15530414-Collagen,
pubmed-meshheading:15530414-Dose-Response Relationship, Drug,
pubmed-meshheading:15530414-Gene Expression,
pubmed-meshheading:15530414-Glycosaminoglycans,
pubmed-meshheading:15530414-Growth Differentiation Factor 5,
pubmed-meshheading:15530414-Humans,
pubmed-meshheading:15530414-Mesenchymal Stem Cells,
pubmed-meshheading:15530414-Transforming Growth Factor beta,
pubmed-meshheading:15530414-Transforming Growth Factor beta1
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pubmed:year |
2004
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pubmed:articleTitle |
Cartilage-derived morphogenetic protein-1 promotes the differentiation of mesenchymal stem cells into chondrocytes.
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pubmed:affiliation |
Peking University Stem Cell Research Center, Peking University Health Science Center, 38 Xue Yuan Road, Beijing 100083, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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