Linked Life Data

15529862

Source:http://linkedlifedata.com/resource/pubmed/id/15529862

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2004-11-8
pubmed:abstractText
A study of temporal and quantitative characteristics of inhibition of replication of Venezuelan equine encephalomyelitis (VEE) virus, strain TC-83, in Vero and CPE on PK cells showed purified polyclonal rabbit antibodies to human recombinant laminin-binding protein (LBP) to be able to block completely the development of cytopathic effect (CPE) in such cells, when infected with 10(7) CPE60. The extent of VEE infection inhibition in Vero was in direct proportion to a concentration of specific antibodies within a range of 0.44-3 microg/100 microl. When antibodies were added to Vero cells after they were infected, there was a gradual attenuation of the inhibition effect, which stopped almost completely 9 hours after the antibodies were placed. Inhibition was effective at 4 degrees C and 37 degrees C. A lack of synthesis of viral glycoprotein E2 in Vero cells infected in the presence of antibodies to LBP is an extra argument proving that the VEE replication is inhibited at early infection stages. The data obtained demonstrated the general LBP significance for the penetration of VEE into mammalian cells and the related importance of designing new antiviral drugs against alpha-viral infection, which are based on blocking the mechanism of receptor penetration of the virus into the cell.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0507-4088
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
32-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15529862-Animals, pubmed-meshheading:15529862-Cell Line, pubmed-meshheading:15529862-Cercopithecus aethiops, pubmed-meshheading:15529862-Dose-Response Relationship, Drug, pubmed-meshheading:15529862-Drug Design, pubmed-meshheading:15529862-Encephalitis Virus, Venezuelan Equine, pubmed-meshheading:15529862-Encephalomyelitis, Venezuelan Equine, pubmed-meshheading:15529862-Humans, pubmed-meshheading:15529862-Immune Sera, pubmed-meshheading:15529862-Immunization, Passive, pubmed-meshheading:15529862-Rabbits, pubmed-meshheading:15529862-Receptors, Laminin, pubmed-meshheading:15529862-Recombinant Proteins, pubmed-meshheading:15529862-Temperature, pubmed-meshheading:15529862-Vero Cells, pubmed-meshheading:15529862-Viral Envelope Proteins, pubmed-meshheading:15529862-Virus Replication
pubmed:articleTitle
[Inhibition of replication of Venezuelan equine encephalomyelitis with polyclonal antibodies to laminin-binding protein].
pubmed:publicationType
Journal Article, Comparative Study, English Abstract, Research Support, Non-U.S. Gov't