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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-12-22
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pubmed:abstractText |
Different molecular forms of prostate-specific antigen (PSA) have been used to differentiate between benign prostatic hyperplasia and prostate cancer. Detecting PSA bound to endogenous inhibitors such as alpha(1)-antichymotrypsin (ACT) and alpha(2)-macroglobulin (alpha(2)M) is often difficult because of epitope masking or sensitivity problems. Here we report the characterization of four novel mouse monoclonal antibodies (mabs) obtained by immunization with PSA-alpha(2)M complexes. Their ability to detect free PSA and PSA-inhibitor complexes was shown, and their epitopes were analyzed by phage display technology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0009-9147
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
84-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15528296-Animals,
pubmed-meshheading:15528296-Antibodies, Monoclonal,
pubmed-meshheading:15528296-Binding, Competitive,
pubmed-meshheading:15528296-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15528296-Epitopes,
pubmed-meshheading:15528296-Female,
pubmed-meshheading:15528296-Immunoassay,
pubmed-meshheading:15528296-Mice,
pubmed-meshheading:15528296-Mice, Inbred BALB C,
pubmed-meshheading:15528296-Models, Molecular,
pubmed-meshheading:15528296-Peptide Library,
pubmed-meshheading:15528296-Prostate-Specific Antigen,
pubmed-meshheading:15528296-Protein Binding,
pubmed-meshheading:15528296-alpha-Macroglobulins
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pubmed:year |
2005
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pubmed:articleTitle |
Characterization of novel monoclonal antibodies for prostate-specific antigen (PSA) with potency to recognize PSA bound to alpha 2-macroglobulin.
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pubmed:affiliation |
Institute of Biochemistry, University of Leipzig, Liebigstrasse 16, 04103 Leipzig, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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