Source:http://linkedlifedata.com/resource/pubmed/id/15528159
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2004-11-5
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pubmed:abstractText |
We previously demonstrated that low-dose radiation conditioning impairs murine hematopoietic stem cell function, permitting engraftment of syngeneic fresh and transduced marrow cells. In this study, we directly examined the ability of low-dose radiation conditioning to permit engraftment of transduced long-term repopulating cells in murine X-linked chronic granulomatous disease (X-CGD), which closely mimics the human disease. X-CGD mice conditioned with 160 cGy were transplanted with 20 x 10(6) MSCV-m91Neo-transduced syngeneic X-CGD marrow cells. The presence of oxidase-positive neutrophils in two independent cohorts of transplanted 160-cGy-conditioned X-CGD recipients was determined by nitroblue tetrazolium testing. Transplanted X-CGD mice (n = 9 total) displayed 1-17% oxidase-positive neutrophils 6-16 months post-transplant. Retroviral marking and NADPH-oxidase-positive neutrophils persisted through serial transplantation, verifying that stem cells were transduced. These results establish that low-dose radiation conditioning results in durable engraftment of low but potentially clinically relevant numbers of functionally reconstituted blood cells in a murine model of X-CGD.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1079-9796
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
365-71
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15528159-Animals,
pubmed-meshheading:15528159-Gene Therapy,
pubmed-meshheading:15528159-Granulomatous Disease, Chronic,
pubmed-meshheading:15528159-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:15528159-Hematopoietic Stem Cells,
pubmed-meshheading:15528159-Membrane Glycoproteins,
pubmed-meshheading:15528159-Mice,
pubmed-meshheading:15528159-Mice, Knockout,
pubmed-meshheading:15528159-NADPH Oxidase,
pubmed-meshheading:15528159-Transplantation Conditioning,
pubmed-meshheading:15528159-Whole-Body Irradiation
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pubmed:articleTitle |
Stable long-term gene correction with low-dose radiation conditioning in murine X-linked chronic granulomatous disease.
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pubmed:affiliation |
Herman B. Wells Center for Pediatric Research, James Whitcomb Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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