Linked Life Data

15527972

Source:http://linkedlifedata.com/resource/pubmed/id/15527972

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-11-5
pubmed:databankReference
pubmed:abstractText
We cloned cDNA encoding chicken HIRA, a homolog of Saccharomyces cerevisiae transcriptional corepressors Hir1p and Hir2p, possessing seven WD dipeptide motifs and a LXXLL motif in its N-terminal and C-terminal regions, respectively. It binds to CAF-1p48, HDAC-1 and 2, but not to CAF-1p60, p46 polypeptide and HDAC-3. The immunoprecipitation experiment involving truncated and missense mutants of HIRA and CAF-1p48 revealed not only that even one of seven WD dipeptide motifs in the N-terminal half of HIRA are necessary for the interaction with CAF-1p48, but also that those of CAF-1p48 are necessary for the interaction with HIRA. These findings indicate that the proper propeller structures of both HIRA and CAF-1p48 are necessary for their in vitro interaction. The immunoprecipitation experiment involving truncated and missense mutants of HIRA and HDAC-2 revealed that the LXXLL motif in the C-terminal half of HIRA and a C-terminal region of HDAC-2 are necessary for their in vitro interaction. Moreover, the WD dipeptide motifs and LXXLL motif of HIRA are essential for the interaction with CAF-1p48 and HDAC-2 in vivo. Taken together, these results indicate that HIRA should participate differentially in a number of DNA-utilizing processes through interactions of its distinct regions with these proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
342
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-36
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15527972-Amino Acid Motifs, pubmed-meshheading:15527972-Amino Acid Sequence, pubmed-meshheading:15527972-Animals, pubmed-meshheading:15527972-Binding Sites, pubmed-meshheading:15527972-Blotting, Western, pubmed-meshheading:15527972-COS Cells, pubmed-meshheading:15527972-Cell Cycle Proteins, pubmed-meshheading:15527972-Cercopithecus aethiops, pubmed-meshheading:15527972-Chickens, pubmed-meshheading:15527972-Chromatin Assembly Factor-1, pubmed-meshheading:15527972-Chromosomal Proteins, Non-Histone, pubmed-meshheading:15527972-Cloning, Molecular, pubmed-meshheading:15527972-DNA, Complementary, pubmed-meshheading:15527972-DNA-Binding Proteins, pubmed-meshheading:15527972-Histone Deacetylase 2, pubmed-meshheading:15527972-Histone Deacetylases, pubmed-meshheading:15527972-Immunoprecipitation, pubmed-meshheading:15527972-Molecular Sequence Data, pubmed-meshheading:15527972-Mutation, pubmed-meshheading:15527972-Plasmids, pubmed-meshheading:15527972-Protein Binding, pubmed-meshheading:15527972-Repressor Proteins, pubmed-meshheading:15527972-Sequence Analysis, DNA, pubmed-meshheading:15527972-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
WD dipeptide motifs and LXXLL motif of chicken HIRA are essential for interactions with the p48 subunit of chromatin assembly factor-1 and histone deacetylase-2 in vitro and in vivo.
pubmed:affiliation
Department of Life Science, Frontier Science Research Center, Miyazaki Medical College, University of Miyazaki, 5200, Kihara, Kiyotake, Miyazaki 889-1692, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't