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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 1
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pubmed:dateCreated |
2005-3-21
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pubmed:abstractText |
A sequence-specific modification of the human 5.8 S rRNA in isolated 60 S subunits, non-programmed 80 S ribosomes and ribosomes complexed with mRNA and tRNAs was studied with the use of a derivative of the nonaribonucleotide UCUGUGUUU bearing a perfluorophenylazide group on the C-5 atom of the 5'-terminal uridine. Part of the oligonucleotide moiety of the derivative was complementary to the 5.8 S rRNA sequence ACACA in positions 82-86 flanked by two guanines at the 5'-terminus. The target for the cross-linking was identified as nucleotide G89 on the 5.8 S RNA. In addition, several ribosomal proteins were modified by the oligonucleotide derivative bound to the 5.8 S rRNA and proteins L6 and L8 were among them. Application of these results to known cryo-electron microscopy images of eukaryotic 60 S subunits made it possible to suggest that the central part of the 5.8 S rRNA containing the sequence 82-86 and proteins L6 and L8 are located at the base of the L1 stalk of the 60 S subunit. The efficacy of cross-linking in non-programmed 80 S ribosomes was much lower than in isolated 60 S subunits and in programmed 80 S ribosomes. We suggest that the difference in the accessibilities of the central part of the 5.8 S rRNA in the programmed and non-programmed 80 S ribosomes is caused by a conformational switch that seems to be required to dissociate the 80 S ribosomes into the subunits after termination of translation to allow initiation of translation of a new template.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15527424-10562562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15527424-10606275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15527424-10835368,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15527424-9608938
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1470-8728
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
387
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-45
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15527424-Base Sequence,
pubmed-meshheading:15527424-Cross-Linking Reagents,
pubmed-meshheading:15527424-Gene Rearrangement,
pubmed-meshheading:15527424-Humans,
pubmed-meshheading:15527424-Molecular Sequence Data,
pubmed-meshheading:15527424-Nucleic Acid Conformation,
pubmed-meshheading:15527424-RNA, Ribosomal, 5.8S,
pubmed-meshheading:15527424-Ribonucleotides,
pubmed-meshheading:15527424-Ribosomal Proteins,
pubmed-meshheading:15527424-Ribosomes
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pubmed:year |
2005
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pubmed:articleTitle |
The central part of the 5.8 S rRNA is differently arranged in programmed and free human ribosomes.
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pubmed:affiliation |
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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