Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2004-11-4
pubmed:abstractText
The S. pombe Cdc14-related phosphatase Clp1p/Flp1p regulates G2/M transition by antagonizing CDK activity and is essential for coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. At the G2/M transition, Clp1p/Flp1p is released from the nucleolus and SPB and distributes throughout the nucleus to the spindle and the contractile ring. This early relocalization is analogous to vertebrate Cdc14 homologs and stands in contrast to S. cerevisiae Cdc14p, which is not released from the nucleolus until metaphase/anaphase transition. Here, we report that Clp1p/Flp1p localizes to kinetochores in prometaphase and functions in chromosome segregation, since deletion of clp1/flp1 causes cosegregation of sister chromatids, when sister kinetochores are prone to mono-orientation. Genetic, cytological, and biochemical experiments suggest that Clp1p/Flp1p functions together with Aurora kinase at kinetochores. Together, these results suggest that Clp1p/Flp1p has a role in repairing mono-orientation of sister kinetochores.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Alexa Fluor 488 carboxylic acid..., http://linkedlifedata.com/resource/pubmed/chemical/Alexa594, http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor..., http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes, http://linkedlifedata.com/resource/pubmed/chemical/Organic Chemicals, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Schizosaccharomyces pombe Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Succinimides, http://linkedlifedata.com/resource/pubmed/chemical/aurora kinase
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1534-5807
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
755-62
pubmed:dateRevised
2011-7-11
pubmed:meshHeading
pubmed-meshheading:15525536-Blotting, Western, pubmed-meshheading:15525536-Cell Cycle Proteins, pubmed-meshheading:15525536-Cell Nucleolus, pubmed-meshheading:15525536-Chromatids, pubmed-meshheading:15525536-Chromosomes, pubmed-meshheading:15525536-Cyclin-Dependent Kinase Inhibitor p21, pubmed-meshheading:15525536-Cytokinesis, pubmed-meshheading:15525536-Fluorescent Dyes, pubmed-meshheading:15525536-Gene Deletion, pubmed-meshheading:15525536-Gene Expression Regulation, Enzymologic, pubmed-meshheading:15525536-Kinetochores, pubmed-meshheading:15525536-Microscopy, Fluorescence, pubmed-meshheading:15525536-Mitotic Spindle Apparatus, pubmed-meshheading:15525536-Organic Chemicals, pubmed-meshheading:15525536-Precipitin Tests, pubmed-meshheading:15525536-Protein-Serine-Threonine Kinases, pubmed-meshheading:15525536-Saccharomyces cerevisiae Proteins, pubmed-meshheading:15525536-Schizosaccharomyces, pubmed-meshheading:15525536-Schizosaccharomyces pombe Proteins, pubmed-meshheading:15525536-Succinimides
pubmed:year
2004
pubmed:articleTitle
The S. pombe Cdc14-like phosphatase Clp1p regulates chromosome biorientation and interacts with Aurora kinase.
pubmed:affiliation
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester, MA 01605, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.