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pubmed-article:15524198rdf:typepubmed:Citationlld:pubmed
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pubmed-article:15524198pubmed:issue10lld:pubmed
pubmed-article:15524198pubmed:dateCreated2004-11-4lld:pubmed
pubmed-article:15524198pubmed:abstractTextA very simple flow-through sensor is presented for the determination of the intense sweetener aspartame in low-calorie and dietary products. The sensor is implemented in a monochannel flow-injection system with UV spectrophotometric detection using a Sephadex CM-C25 cationic exchanger packed 20 mm high in a flow cell. This method is based on the transient retention of a cationic species of the sweetener on the solid phase when a pH 5.0 acetic acid sodium acetate buffer (0.01 M) is used as a carrier (2.6 mL(-1) min). The carrier itself elutes the analyte from the solid support, regenerating a sensing zone. Aspartame was determined by measuring its intrinsic absorbance at 219 nm at its residence time without any derivatization. Calibration graphs were linear over the range of 5.0 - 600.0 microg mL(-1) with an RSD of 0.55% (peak height). This sweetener was determined in several samples by measuring the height or peak area, obtaining recoveries ranging between 95 - 101% and 97.5 - 101%, respectively. The procedure was validated for its use in the determination of aspartame in low-calorie and dietary products, giving reproducible and accurate results.lld:pubmed
pubmed-article:15524198pubmed:languageenglld:pubmed
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pubmed-article:15524198pubmed:statusMEDLINElld:pubmed
pubmed-article:15524198pubmed:monthOctlld:pubmed
pubmed-article:15524198pubmed:issn0910-6340lld:pubmed
pubmed-article:15524198pubmed:authorpubmed-author:Capitán-Vallv...lld:pubmed
pubmed-article:15524198pubmed:authorpubmed-author:ValenciaM CMClld:pubmed
pubmed-article:15524198pubmed:authorpubmed-author:NicolásE...lld:pubmed
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pubmed-article:15524198pubmed:volume20lld:pubmed
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pubmed-article:15524198pubmed:pagination1437-42lld:pubmed
pubmed-article:15524198pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:15524198pubmed:year2004lld:pubmed
pubmed-article:15524198pubmed:articleTitleFlow-through spectrophotometric sensor for the determination of aspartame in low-calorie and dietary products.lld:pubmed
pubmed-article:15524198pubmed:affiliationDepartment of Analytical Chemistry, Research Group Solid Phase Spectrometry, Faculty of Sciences, University of Granada, Avda, Fuentenueva s/n, E-18071 Granada, Spain. lcapitan@ugr.eslld:pubmed
pubmed-article:15524198pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15524198pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed