Linked Life Data

15522613

Source:http://linkedlifedata.com/resource/pubmed/id/15522613

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2004-11-3
pubmed:abstractText
Extensive use of insecticides on cotton in the mid-South has prompted resistance development in the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois). A field population of tarnished plant bugs in Mississippi with 11-fold higher resistance to malathion was used to examine how gene regulation conferred resistance to this organophosphate insecticide. In laboratory bioassays, synergism by the esterase inhibitors S,S,S,-tributylphosphorotrithioate (DEF) and triphenylphosphate (TPP) effectively abolished resistance and increased malathion toxicity by more than 80%. Esterase activities were compared in vitro between malathion susceptible and resistant (selected) strains. More than 6-, 3- and 10-fold higher activities were obtained with the resistant strain using alpha-naphthyl acetate, beta-naphthyl acetate, and p-nitrophenyl acetate, respectively. Up to 95% and 89% of the esterase activity in the susceptible and resistant strains, respectively, was inhibited by 1 mM DEF. Inhibition of esterase activity up to 75% and 85% in the susceptible and resistant strains, respectively, was obtained with 0.03 mM TPP. Esterase activities in field populations increased by up to 5.4-fold during the fall season. The increase was synchronized with movement of the insect into cotton where exposure to pesticides occurred. Esterase cDNA was cloned and sequenced from both malathion susceptible and resistant strains. The 1818-nucleotide cDNA contained a 1710-bp open reading frame coding a 570 amino acid protein which was similar to many insect esterases conferring organophosphate resistance. No amino acid substitution was observed between susceptible and resistant strains, indicating that esterase gene mutation was not involved in resistance development in the resistant strain in Mississippi. Further examination of esterase gene expression levels using quantitative RT-PCR revealed that the resistant strain had a 5.1-fold higher level of esterase mRNA than the susceptible strain. The results of this study indicated that up-regulation of the esterase gene appeared to be related to the development of resistance in the tarnished plant bug.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0965-1748
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1175-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15522613-Amino Acid Sequence, pubmed-meshheading:15522613-Animals, pubmed-meshheading:15522613-Base Sequence, pubmed-meshheading:15522613-DNA, Complementary, pubmed-meshheading:15522613-Enzyme Inhibitors, pubmed-meshheading:15522613-Esterases, pubmed-meshheading:15522613-Gene Expression, pubmed-meshheading:15522613-Genes, Insect, pubmed-meshheading:15522613-Gossypium, pubmed-meshheading:15522613-Heteroptera, pubmed-meshheading:15522613-Insecticide Resistance, pubmed-meshheading:15522613-Malathion, pubmed-meshheading:15522613-Mississippi, pubmed-meshheading:15522613-Molecular Sequence Data, pubmed-meshheading:15522613-Phosphoric Acid Esters, pubmed-meshheading:15522613-RNA, Messenger, pubmed-meshheading:15522613-Sequence Homology, Amino Acid, pubmed-meshheading:15522613-Thiophosphoric Acid Esters
pubmed:year
2004
pubmed:articleTitle
Enhanced esterase gene expression and activity in a malathion-resistant strain of the tarnished plant bug, Lygus lineolaris.
pubmed:affiliation
Jamie Whitten Delta States Research Center, ARS-USDA, Stoneville, PO Box 346, Mississippi 38776, USA. yczhu@ars.usda.gov
pubmed:publicationType
Journal Article