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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1992-4-28
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pubmed:abstractText |
The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galp beta 1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galp beta 1-4(Manp alpha 1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an alpha-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as beta. The complete structure of the core is thus PO4-6Galp alpha 1-6Galp alpha 1-3Galf beta 1-3[Glcp alpha 1-PO4-6]Manp alpha 1-3Manp alpha 1-4GlcN alpha 1-. This further clarification of the structure of lipophosphoglycan may prove beneficial in determining the structure-function relationships of this highly unusual glycoconjugate.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6829-33
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pubmed:dateRevised |
2009-9-29
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pubmed:meshHeading |
pubmed-meshheading:1551889-Animals,
pubmed-meshheading:1551889-Carbohydrate Sequence,
pubmed-meshheading:1551889-Chromatography, High Pressure Liquid,
pubmed-meshheading:1551889-Glycosphingolipids,
pubmed-meshheading:1551889-Hydrolysis,
pubmed-meshheading:1551889-Leishmania donovani,
pubmed-meshheading:1551889-Magnetic Resonance Spectroscopy,
pubmed-meshheading:1551889-Molecular Sequence Data,
pubmed-meshheading:1551889-Spectrometry, Mass, Fast Atom Bombardment
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pubmed:year |
1992
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pubmed:articleTitle |
Refined structure of the lipophosphoglycan of Leishmania donovani.
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pubmed:affiliation |
Department of Biochemistry, University of Dundee, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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