Source:http://linkedlifedata.com/resource/pubmed/id/15517343
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2005-2-28
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| pubmed:abstractText |
Inorganic phosphate (P(i)) is reabsorbed in the renal proximal tubule mainly via the type-IIa sodium-phosphate cotransporter (NaPi-IIa). This protein is regulated tightly by different factors, among them dietary P(i) intake and parathyroid hormone (PTH). A number of PDZ-domain-containing proteins have been shown to interact with NaPi-IIa in vitro, such as Na(+)/H(+) exchanger-3 regulatory factor-1 (NHERF1) and PDZK1. PDZK1 is highly abundant in kidney and co-localizes with NaPi-IIa in the brush border membrane of proximal tubules. Recently, a knock-out mouse model for PDZK1 (Pdzk1(-/-)) has been generated, allowing the role of PDZK1 in the expression and regulation of the NaPi-IIa cotransporter to be examined in in vivo and in ex vivo preparations. The localization of NaPi-IIa and other proteins interacting with PDZK1 in vitro [Na(+)/H(+) exchanger (NHE3), chloride-formate exchanger (CFEX)/putative anion transporter-1 (PAT1), NHERF1] was not altered in Pdzk1(-/-) mice. The abundance of NaPi-IIa adapted to acute and chronic changes in dietary P(i) intake, but steady-state levels of NaPi-IIa were reduced in Pdzk1(-/-) under a P(i) rich diet. This was paralleled by a higher urinary fractional P(i) excretion. The abundance of the anion exchanger CFEX/PAT1 (SLC26A6) was also reduced. In contrast, NHERF1 abundance increased in the brush border membrane of Pdzk1(-/-) mice fed a high-P(i) diet. Acute regulation of NaPi-IIa by PTH in vivo and by PTH and activators of protein kinases A, C and G (PKA, PKC and PKG) in vitro (kidney slice preparation) was not altered in Pdzk1(-/-) mice. In conclusion, loss of PDZK1 did not result in major changes in proximal tubule function or NaPi-IIa regulation. However, under a P(i)-rich diet, loss of PDZK1 reduced NaPi-IIa abundance indicating that PDZK1 may play a role in the trafficking or stability of NaPi-IIa under these conditions.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Slc34a1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Phosphate Cotransporter...,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Phosphate Cotransporter...,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/sodium-hydrogen exchanger...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0031-6768
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
449
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
392-402
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:15517343-Animals,
pubmed-meshheading:15517343-Blotting, Western,
pubmed-meshheading:15517343-Cytoskeletal Proteins,
pubmed-meshheading:15517343-Diet,
pubmed-meshheading:15517343-Female,
pubmed-meshheading:15517343-Immunohistochemistry,
pubmed-meshheading:15517343-Kidney,
pubmed-meshheading:15517343-Kidney Tubules, Proximal,
pubmed-meshheading:15517343-Male,
pubmed-meshheading:15517343-Membrane Proteins,
pubmed-meshheading:15517343-Mice,
pubmed-meshheading:15517343-Mice, Knockout,
pubmed-meshheading:15517343-Microvilli,
pubmed-meshheading:15517343-Phosphates,
pubmed-meshheading:15517343-Phosphoproteins,
pubmed-meshheading:15517343-Protein Kinases,
pubmed-meshheading:15517343-Sodium-Hydrogen Antiporter,
pubmed-meshheading:15517343-Sodium-Phosphate Cotransporter Proteins,
pubmed-meshheading:15517343-Sodium-Phosphate Cotransporter Proteins, Type IIa,
pubmed-meshheading:15517343-Symporters
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| pubmed:year |
2005
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| pubmed:articleTitle |
Expression and regulation of the renal Na/phosphate cotransporter NaPi-IIa in a mouse model deficient for the PDZ protein PDZK1.
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| pubmed:affiliation |
Institute of Physiology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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