Source:http://linkedlifedata.com/resource/pubmed/id/15514467
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2004-10-29
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pubmed:abstractText |
The effect of developmental stage on the survival of bovine somatic cell nuclear-transferred blastocysts after freezing and thawing was evaluated. We also investigated how freezing affects nuclear-transferred (NT) embryos and in vitro fertilized (IVF) bovine embryos. Advanced-stage bovine NT blastocysts survived freezing better than early-stage NT blastocysts (86 vs 14%). The trend was similar with IVF embryos (87 vs 30%). At the stages tested, there was no significant difference in the survivability of NT and IVF embryos from advanced (86 vs 87%) or early-stage blastocysts (14 vs 30%). The average survival rate did not differ between NT and IVF bovine embryos (50 vs 51%). The higher survival rate of advanced-stage blastocysts compared to early-stage blastocysts in NT and IVF bovine embryos might be due to their higher cell number. In NT (128 +/- 25 vs 53 +/- 20) and IVF (128 +/- 29 vs 75 +/- 22) groups, advanced-stage blastocysts contained a significantly higher total cell number than early-stage blastocysts. There was no difference in total cell number between advanced-stage NT and IVF blastocysts (128 +/- 25 vs 128 +/- 29), however, early-stage NT and IVF blastocysts (53 +/- 20 vs 75 +/- 22) differed significantly.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0916-8818
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
593-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15514467-Animals,
pubmed-meshheading:15514467-Blastomeres,
pubmed-meshheading:15514467-Cattle,
pubmed-meshheading:15514467-Cell Survival,
pubmed-meshheading:15514467-Cryopreservation,
pubmed-meshheading:15514467-Ethylene Glycol,
pubmed-meshheading:15514467-Fertilization in Vitro,
pubmed-meshheading:15514467-Male,
pubmed-meshheading:15514467-Nuclear Transfer Techniques,
pubmed-meshheading:15514467-Zona Pellucida
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pubmed:year |
2004
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pubmed:articleTitle |
Cryopreservation of bovine somatic cell nuclear-transferred blastocysts: effect of developmental stage.
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pubmed:affiliation |
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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