Linked Life Data

15507391

Source:http://linkedlifedata.com/resource/pubmed/id/15507391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-10-27
pubmed:abstractText
Fusion of human dendritic cells (DC) with tumor cells is an effective approach for delivering tumor antigens to DC, and DC/tumor fusion cells are potent stimulators of autologous T cells. However, the integration and morphology of DC/tumor fusion cells has not been examined. In the present study, we fused patient-derived DC to autologous breast or ovarian carcinoma cells. The fusion cells possessed the properties of both parent cells. After fusion, the cytoplasm of the two cells was integrated, whereas their nuclei remained separate entities. Colocalization of MUC1 peptide and HLA-DR molecules was observed on fusion cells under the immunoelectron microscope. Coculture of patient-derived peripheral blood mononuclear cells (PBMC) with DC/tumor fusion cells resulted in activation of CD4 and CD8 T cells as assessed by IFN-gamma secretion, HLA-A*0201-MUC1 tetramer, and standard cytotoxic T lymphocyte (CTL) assays. The present study provides first evidence of integration of human DC and tumor cells and links their properties to T cell activation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1521-6616
pubmed:author
pubmed:issnType
Print
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15507391-Antigens, pubmed-meshheading:15507391-Breast Neoplasms, pubmed-meshheading:15507391-Cell Fusion, pubmed-meshheading:15507391-Cell Shape, pubmed-meshheading:15507391-Cells, Cultured, pubmed-meshheading:15507391-Dendritic Cells, pubmed-meshheading:15507391-Female, pubmed-meshheading:15507391-Histocompatibility Antigens Class I, pubmed-meshheading:15507391-Histocompatibility Antigens Class II, pubmed-meshheading:15507391-Humans, pubmed-meshheading:15507391-Lymphocyte Activation, pubmed-meshheading:15507391-Membrane Proteins, pubmed-meshheading:15507391-Microscopy, Electron, Scanning, pubmed-meshheading:15507391-Microscopy, Electron, Transmission, pubmed-meshheading:15507391-Ovarian Neoplasms, pubmed-meshheading:15507391-T-Lymphocytes, pubmed-meshheading:15507391-T-Lymphocytes, Cytotoxic
pubmed:year
2004
pubmed:articleTitle
Dendritic cells fused with human cancer cells: morphology, antigen expression, and T cell stimulation.
pubmed:affiliation
Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't