Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2004-11-16
pubmed:abstractText
In this report we examined overlap between superantigen (SAg) and Toll-like receptor 4 (TLR4) stimulation of the innate immune system. Before in vivo stimulation we found that mouse splenic DCs expressed unexpectedly low levels of surface TLR4 compared to macrophages. In response to LPS, TLR4 gene expression in fractionated spleen cells was downregulated. By comparison, surface TLR4 staining with the Sa15-21 mAb showed little downregulation, and the anti-TLR4 MTS510 mAb showed decreased staining, suggesting that LPS was bound to TLR4 at the time points examined. Interestingly, SAg stimulation induced decreased TLR4 staining as measured by the MTS510 mAb, even though the TLR4 gene was not downregulated. Nevertheless, LPS potently induced DCs to produce TNF and IL-12, but SAg did not, even though they efficiently activated DCs. Notwithstanding, in vivo stimulation with staphylococcal enterotoxin SAg conditioned the innate immune system to hyper-respond to various pathogen-associated molecular patterns (PAMPs). Specifically, pre-priming with SAg enhanced LPS-mediated DC synthesis of TNF and IL-12. Thus, SAgs may exert their pathogenesis on the host by conditioning DCs, in a T cell activation dependent manner to potentiate responses to PAMPs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1751-60
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
pubmed-meshheading:15504761-Animals, pubmed-meshheading:15504761-Dendritic Cells, pubmed-meshheading:15504761-Enterotoxins, pubmed-meshheading:15504761-Gene Expression, pubmed-meshheading:15504761-Immunity, Innate, pubmed-meshheading:15504761-Interleukin-12, pubmed-meshheading:15504761-Lipopolysaccharides, pubmed-meshheading:15504761-Lymphocyte Activation, pubmed-meshheading:15504761-Membrane Glycoproteins, pubmed-meshheading:15504761-Mice, pubmed-meshheading:15504761-Protein Subunits, pubmed-meshheading:15504761-Receptors, Cell Surface, pubmed-meshheading:15504761-Spleen, pubmed-meshheading:15504761-Staphylococcus aureus, pubmed-meshheading:15504761-Superantigens, pubmed-meshheading:15504761-T-Lymphocytes, pubmed-meshheading:15504761-Toll-Like Receptor 4, pubmed-meshheading:15504761-Toll-Like Receptors, pubmed-meshheading:15504761-Tumor Necrosis Factor-alpha
pubmed:year
2004
pubmed:articleTitle
Staphylococcal enterotoxins condition cells of the innate immune system for Toll-like receptor 4 stimulation.
pubmed:affiliation
Division of Immunology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.