Linked Life Data

15502207

Source:http://linkedlifedata.com/resource/pubmed/id/15502207

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-10-25
pubmed:abstractText
The original 32P-postlabeling method developed by Randerath and his colleagues has been modified to detect a single type of adduct as a single spot in thin-layer chromatography (TLC), because some types of adducts gave multiple adduct spots by the original method. In the remodified methods, DNA is first digested with micrococcal nuclease and phophodiesterase II and then labeled with [gamma-32P]ATP under standard or adduct-intensification conditions. Since the labeled digest includes adducted mono-, di-, and/or oligo-deoxynucleotides, it is further treated with phosphatase and phosphodiesterase prior to TLC. The labeled digest is treated with nuclease P1 (NP1) in method I, and with T4 polynucleotide kinase and NP1 in method II, and then with phosphodiesterase I in both cases, and subjected to TLC. The advantage of these methods is that the number of adduct species formed can be estimated by TLC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
291
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-9
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Modification of the 32P-postlabeling method to detect a single adduct species as a single spot.
pubmed:affiliation
Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan.
pubmed:publicationType
Journal Article