| pubmed-article:15495251 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15495251 | lifeskim:mentions | umls-concept:C0019046 | lld:lifeskim |
| pubmed-article:15495251 | lifeskim:mentions | umls-concept:C0001898 | lld:lifeskim |
| pubmed-article:15495251 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:15495251 | lifeskim:mentions | umls-concept:C0369765 | lld:lifeskim |
| pubmed-article:15495251 | lifeskim:mentions | umls-concept:C0205251 | lld:lifeskim |
| pubmed-article:15495251 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:15495251 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:15495251 | pubmed:dateCreated | 2004-10-25 | lld:pubmed |
| pubmed-article:15495251 | pubmed:abstractText | Hemoglobin (Hb) Bassett, an abnormal Hb variant with a markedly reduced oxygen affinity, was discovered in a Caucasian (Anglo-Saxon) male child who experienced episodes of cyanosis. Cation-exchange and reversed-phase (RP) high-performance liquid chromatography (HPLC) showed that the patient has an abnormal Hb, with a mutation in the alpha-globin. Tryptic peptide digest of the abnormal alpha-globin with subsequent HPLC analysis revealed abnormal elution of the alpha-T11 peptide. Further studies with Edman sequencing and electrospray mass spectrometry of tryptic peptide alpha-T11, as well as structural analysis by X-ray crystallography revealed an Asp-->Ala substitution at the alpha94 (G1) position, a match for Hb Bassett. Detailed functional studies showed that this Hb variant had a markedly reduced oxygen affinity (P(50) at pH 7.0 = 22 mmHg; Hb A P(50) = 10.5 mmHg), reduced Bohr effect (-0.26 compared to - 0.54 in Hb A), and low subunit cooperativity (n = 1.4, compared to 2.6 in Hb A). X-ray crystallography results explain the probable effects of the structural modification on the oxygen-binding properties of this Hb variant. | lld:pubmed |
| pubmed-article:15495251 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15495251 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15495251 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15495251 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15495251 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15495251 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15495251 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15495251 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15495251 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15495251 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15495251 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15495251 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:15495251 | pubmed:issn | 0361-8609 | lld:pubmed |
| pubmed-article:15495251 | pubmed:author | pubmed-author:SafoMartin... | lld:pubmed |
| pubmed-article:15495251 | pubmed:author | pubmed-author:AbrahamDonald... | lld:pubmed |
| pubmed-article:15495251 | pubmed:author | pubmed-author:SantacroceRos... | lld:pubmed |
| pubmed-article:15495251 | pubmed:author | pubmed-author:AsakuraToshio... | lld:pubmed |
| pubmed-article:15495251 | pubmed:author | pubmed-author:AbdulmalikOsh... | lld:pubmed |
| pubmed-article:15495251 | pubmed:author | pubmed-author:LakkaVinaysag... | lld:pubmed |
| pubmed-article:15495251 | pubmed:author | pubmed-author:LernerNorma... | lld:pubmed |
| pubmed-article:15495251 | pubmed:author | pubmed-author:OchotorenaJos... | lld:pubmed |
| pubmed-article:15495251 | pubmed:author | pubmed-author:DaikhinEvguen... | lld:pubmed |
| pubmed-article:15495251 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15495251 | pubmed:volume | 77 | lld:pubmed |
| pubmed-article:15495251 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15495251 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15495251 | pubmed:pagination | 268-76 | lld:pubmed |
| pubmed-article:15495251 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
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| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
| pubmed-article:15495251 | pubmed:meshHeading | pubmed-meshheading:15495251... | lld:pubmed |
| pubmed-article:15495251 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15495251 | pubmed:articleTitle | Characterization of hemoglobin bassett (alpha94Asp-->Ala), a variant with very low oxygen affinity. | lld:pubmed |
| pubmed-article:15495251 | pubmed:affiliation | Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA. | lld:pubmed |
| pubmed-article:15495251 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15495251 | pubmed:publicationType | Comparative Study | lld:pubmed |
| entrez-gene:3039 | entrezgene:pubmed | pubmed-article:15495251 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15495251 | lld:pubmed |
