Source:http://linkedlifedata.com/resource/pubmed/id/15494490
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0036849,
umls-concept:C0185117,
umls-concept:C0814999,
umls-concept:C0851285,
umls-concept:C1442518,
umls-concept:C1515655,
umls-concept:C1552652,
umls-concept:C1552685,
umls-concept:C1705195,
umls-concept:C1880371,
umls-concept:C2347644,
umls-concept:C2911684
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| pubmed:issue |
9
|
| pubmed:dateCreated |
2004-10-20
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| pubmed:abstractText |
A signal initiated by the newly formed Ag receptor is integrated with microenvironmental cues during T cell development to ensure positive selection of CD4+CD8+ progenitors into functionally mature CD4+ or CD8+ T lymphocytes. During this transition, a survival program is initiated, TCR gene recombination ceases, cells migrate into a new thymic microenvironment, the responsiveness of the Ag receptor is tuned, and the cells commit to a specific T lineage. To determine potential regulators of these processes, we used mRNA microarray analysis to compare gene expression changes in CD4+CD8+ thymocytes from TCR transgenic mice that have received a TCR selection signal with those that had not received a signal. We found 129 genes with expression that changed significantly during positive selection, the majority of which were not previously appreciated. A large number of these changes were confirmed by real-time PCR or flow cytometry. We have combined our findings with gene changes reported in the literature to provide a comprehensive report of the genes regulated during positive selection, and we attempted to assign these genes to positive selection process categories.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
|
| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
173
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5434-44
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:15494490-Animals,
pubmed-meshheading:15494490-Cell Adhesion,
pubmed-meshheading:15494490-Cell Death,
pubmed-meshheading:15494490-Cell Differentiation,
pubmed-meshheading:15494490-Cell Lineage,
pubmed-meshheading:15494490-Cell Movement,
pubmed-meshheading:15494490-Cell Survival,
pubmed-meshheading:15494490-Gene Expression Profiling,
pubmed-meshheading:15494490-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:15494490-Kinetics,
pubmed-meshheading:15494490-Mice,
pubmed-meshheading:15494490-Mice, Inbred C57BL,
pubmed-meshheading:15494490-Mice, Knockout,
pubmed-meshheading:15494490-Mice, Transgenic,
pubmed-meshheading:15494490-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15494490-Receptors, Antigen, T-Cell,
pubmed-meshheading:15494490-Recombination, Genetic,
pubmed-meshheading:15494490-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15494490-T-Lymphocyte Subsets,
pubmed-meshheading:15494490-Thymus Gland,
pubmed-meshheading:15494490-Transcription Factors
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| pubmed:year |
2004
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| pubmed:articleTitle |
The regulated expression of a diverse set of genes during thymocyte positive selection in vivo.
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| pubmed:affiliation |
Center for Immunology, Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Validation Studies
|