Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1992-4-20
|
| pubmed:abstractText |
We have undertaken a detailed analysis of the cis-acting elements that are required for optimal transcription initiation from P2, the major promoter of the murine c-myc gene. We find that three elements contribute to promoter strength, termed ME1a2, E2F and ME1a1 at positions -85, -64 and -46 respectively (relative to P2). Individually the elements are weak, but combined they contribute to full promoter activity, all acting in a positive fashion. The E2F element is the site at which the SV40 large T antigen transactivates the c-myc promoter. However, transactivation requires the presence of the ME1a2 or ME1a1 elements in addition to E2F. By a number of criteria it appears that the ME1a2 and ME1a1 elements bind the same or a very closely related protein (monomer Mr 94,000). It was found that Hela cells contain a novel factor that is capable of binding to the ME1a1 and ME1a2 elements but only in the presence of the protein-dissociating agents deoxycholate or formamide. Finally, the E2F factor binds DNA both as a monomer and as a multiprotein complex; the latter is cell type specific. Both types of bound E2F factor form less stable protein-DNA complexes than the ME1a2/ME1a1 factor.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Polyomavirus Transforming,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Multiprotein Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0950-9232
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:geneSymbol |
c-myc
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
411-21
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1549358-3T3 Cells,
pubmed-meshheading:1549358-Animals,
pubmed-meshheading:1549358-Antigens, Polyomavirus Transforming,
pubmed-meshheading:1549358-Base Sequence,
pubmed-meshheading:1549358-DNA-Binding Proteins,
pubmed-meshheading:1549358-Gene Expression Regulation,
pubmed-meshheading:1549358-Genes, myc,
pubmed-meshheading:1549358-HeLa Cells,
pubmed-meshheading:1549358-Humans,
pubmed-meshheading:1549358-Macromolecular Substances,
pubmed-meshheading:1549358-Mice,
pubmed-meshheading:1549358-Molecular Sequence Data,
pubmed-meshheading:1549358-Multiprotein Complexes,
pubmed-meshheading:1549358-Oligodeoxyribonucleotides,
pubmed-meshheading:1549358-Promoter Regions, Genetic,
pubmed-meshheading:1549358-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1549358-Sequence Alignment,
pubmed-meshheading:1549358-Transcription, Genetic,
pubmed-meshheading:1549358-Transcriptional Activation
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Three distinct elements within the murine c-myc promoter are required for transcription.
|
| pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|