Linked Life Data

15492400

Source:http://linkedlifedata.com/resource/pubmed/id/15492400

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-10-19
pubmed:abstractText
Hematopoietic stem cells (HSCs) constitute a pool of very rare cells able to self-renew, proliferate, and/or differentiate to all the blood cell lineages during the life span. The first murine adult transplantable HSCs appear in the intraembryonic aorta-gonad-mesonephros region at embryonic day (E) 10.5. After E11, these HSCs are thought to seed the liver and then the bone marrow just before birth. So far, many questions concerning the origin, properties, and functionality of these HSCs have not been answered. To address these issues, it is necessary to isolate and purify them. One of the major problems concerning embryonic HSCs, as compared with their adult counterpart, is that they share many markers with endothelial cells at this early stage of development, making their purification very difficult. This review presents the best methods (sorting based on specific antibody staining, transgenic markers, cell cycle) for the purification and isolation of HSCs from the mouse embryo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1543-1894
pubmed:author
pubmed:issnType
Print
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
257-72
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Hematopoietic stem cell enrichment from the AGM region of the mouse embryo.
pubmed:affiliation
Department of Cell Biology and Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review, Research Support, Non-U.S. Gov't