Linked Life Data

15491684

Source:http://linkedlifedata.com/resource/pubmed/id/15491684

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2004-10-19
pubmed:abstractText
Drosophila hsp22 is a member of the small heat shock proteins family (shsps). The hsp22 is expressed in a tissue-general pattern in response to heat stress and during normal aging, and localizes to the mitochondrial matrix, however, its exact function and targets are unknown. Hsp22 was found to be rapidly induced in response to oxidative stress, indicating that hsp22 is also an oxidative stress response gene. To assay for effects of hsp22, a ubiquitous pattern of hsp22 gene expression was generated in young flies using the "tet-on" doxycycline-regulated promoter system. The hsp22 over-expression made flies more sensitive to heat and oxidative stress, while resistance to coumarin poisoning was not affected. Life span was also reduced, particularly at higher culture temperatures. Members of other hsp families have been shown to feedback-inhibit their own expression by interacting with the heat shock transcription factor (HSF) and preventing binding to the HSEs. Induction of hsp22:lacZ and hsp70:lacZ reporter transgenes in response to acute stress was normal in the presence of hsp22 protein over-expression and in old flies, indicating that the negative effects of hsp22 are downstream of the HSF/HSE pathway and the transcriptional heat shock response. The data demonstrate a specific over-expression phenotype for hsp22 and suggest that hsp22 interacts with heat and oxidative stress resistance pathways.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0047-6374
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
651-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15491684-Aging, pubmed-meshheading:15491684-Animals, pubmed-meshheading:15491684-Coumarins, pubmed-meshheading:15491684-Disease Susceptibility, pubmed-meshheading:15491684-Doxycycline, pubmed-meshheading:15491684-Drosophila Proteins, pubmed-meshheading:15491684-Drosophila melanogaster, pubmed-meshheading:15491684-Drug Resistance, pubmed-meshheading:15491684-Gene Expression Regulation, pubmed-meshheading:15491684-Genes, Reporter, pubmed-meshheading:15491684-HSP70 Heat-Shock Proteins, pubmed-meshheading:15491684-Heat Stress Disorders, pubmed-meshheading:15491684-Heat-Shock Proteins, pubmed-meshheading:15491684-Longevity, pubmed-meshheading:15491684-Male, pubmed-meshheading:15491684-Oxidative Stress, pubmed-meshheading:15491684-Paraquat, pubmed-meshheading:15491684-Transcription, Genetic
pubmed:year
2004
pubmed:articleTitle
Doxycycline-regulated over-expression of hsp22 has negative effects on stress resistance and life span in adult Drosophila melanogaster.
pubmed:affiliation
Department of Biological Sciences, University of Southern California, Los Angeles 90089 1340, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.