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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1992-4-22
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pubmed:abstractText |
We have identified difference in protein-DNA interactions associated with the promoter of the mammalian spermatogenesis-specific Pgk-2 gene in expressing and nonexpressing cells, using a band shift assay. We compared DNA-binding activities in nuclear protein extracts from expressing adult testis cells versus nonexpressing prepuberal testis cells and nonexpressing somatic cells. One or two DNA-binding activities were found to be uniquely associated with the expressed state of Pgk-2, while a third appears to be associated with the nonexpressed state. All three of these activities map to a region within the first 40 bp upstream from the core promoter of this gene. The Pgk-2 core promoter lacks a TATA box but contain a GC box and a CAAT box. We show that the GC box binds the ubiquitous transcription factor Sp1 and that the CAAT box binds CTF-1, both of which are present in extracts from all three tissue types tested. These results suggest that tissue-specific transcription of the Pgk-2 gene is associated with changes in protein-DNA interactions occurring within a 40-bp enhancer region and that different arrays of protein-DNA interactions in this region are associated with the actively expressed state of the Pgk-2 gene in spermatocytes and spermatids and with the terminally repressed state of Pgk-2 in somatic cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-1850324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2005904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-20815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2156237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2194667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2300581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2355920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2467742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2482783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2673023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2813402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2823118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2987238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2992804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-2996137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3004739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3024847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3358932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3398920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3446575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3453121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3476205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3529394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3607873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3733754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-3886593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-4040852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-4041012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-6095100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-6187469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-6313230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-6933441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-7286419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-874003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1549104-978173
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1422-31
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1549104-Animals,
pubmed-meshheading:1549104-Base Sequence,
pubmed-meshheading:1549104-Gene Expression Regulation,
pubmed-meshheading:1549104-Male,
pubmed-meshheading:1549104-Mice,
pubmed-meshheading:1549104-Models, Genetic,
pubmed-meshheading:1549104-Molecular Sequence Data,
pubmed-meshheading:1549104-Organ Specificity,
pubmed-meshheading:1549104-Phosphoglycerate Kinase,
pubmed-meshheading:1549104-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1549104-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1549104-Sexual Maturation,
pubmed-meshheading:1549104-Sp1 Transcription Factor,
pubmed-meshheading:1549104-Testis,
pubmed-meshheading:1549104-Transcription, Genetic,
pubmed-meshheading:1549104-Transcription Factors
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pubmed:year |
1992
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pubmed:articleTitle |
Protein-DNA interactions associated with the onset of testis-specific expression of the mammalian Pgk-2 gene.
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pubmed:affiliation |
Division of Reproductive Biology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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