Linked Life Data

15488175

Source:http://linkedlifedata.com/resource/pubmed/id/15488175

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-10-18
pubmed:abstractText
RGS8 was identified as a regulator of G-protein signaling (RGS) protein induced in neuronally differentiated P19 cells. This article presents methods used to estimate the binding activity and selectivity between RGS8 and Galpha subunits. It describes three kinds of in vitro-binding experiments using RGS8 proteins generated by three different techniques: recombinant protein from Escherichia coli, in vitro-translated protein, and protein expressed in cDNA-transfected cultured cells. It also presents methods of the functional analysis of RGS protein using the Xenopus oocyte expression system. Electrophysiological procedures, which were used to examine the effects of RGS8 on Gi- and Gq-mediated responses, are described.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
390
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-48
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Biochemical and electrophysiological analyses of RGS8 function.
pubmed:affiliation
Department of Molecular Cell Signaling, Tokyo Metropolitan Institute for Neuroscience, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't