Linked Life Data

15487890

Source:http://linkedlifedata.com/resource/pubmed/id/15487890

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2004-10-18
pubmed:abstractText
Epidemiological studies have implicated a role for airborne particulates of <2.5 microm diameter in the development/exacerbation of chronic cardiopulmonary disease; however, specific pathogenic mechanisms and the etiological significance of particle physicochemical properties remain unresolved. Using a microporous aluminosilicate zeolite Y as a manifold, we have synthesized 1 microm particulates of pure carbon (C), carbon-iron (C/Fe), and carbon-iron/fluoro-aluminum silicate (C-Fe/F-Al-Si). We have used these particulates, as well as coal fly ash (CFA) and diesel exhaust particulates (DEP), to test the hypotheses that human macrophages treated with particulates elaborate proinflammatory cytokines in quantities sufficient to induce endothelial adhesion molecule expression and that macrophage responses to particulate exposure vary as a function of particulate physicochemical properties. Human monocyte-derived macrophages (Mø) were exposed for 24 h to sublethal concentrations of particulates, at which time phagocytosis was evident from optical microscopy. Human arterial, microvascular, or venous endothelial cells (EC) were treated with clarified supernatants recovered from Mø cultures, stained with fluorescein-conjugated mononclonal antibodies specific for endothelial adhesion molecules intercellular adhesion molecule-1, vascular cell adhesion molecule-1, or E-selectin, and assayed by fluorescence flow cytometry. Data generated by these experiments demonstrate that while supernatants of Mø exposed to CFA and C particulates are relatively ineffective, supernatants from DEP, C/Fe, or C-Fe/F-Al-Si strongly induced adhesion molecule expression on EC, responses which were completely attenuated by antibody with blocking specificity for tumor necrosis factor alpha. Because the only difference between C and C/Fe particulates is the presence of surface iron on C/Fe, these findings suggest particulate-induced oxidative stress as a contributing factor in Mø activation and implicate redox active iron as a major determinant of particulate bioreactivity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0893-228X
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1303-12
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:15487890-Air Pollutants, pubmed-meshheading:15487890-Aluminum Silicates, pubmed-meshheading:15487890-Carbon, pubmed-meshheading:15487890-Cell Adhesion Molecules, pubmed-meshheading:15487890-Cells, Cultured, pubmed-meshheading:15487890-Chemistry, Physical, pubmed-meshheading:15487890-Coal, pubmed-meshheading:15487890-Coal Ash, pubmed-meshheading:15487890-Cytokines, pubmed-meshheading:15487890-Endothelium, Vascular, pubmed-meshheading:15487890-Fluorine Compounds, pubmed-meshheading:15487890-Gasoline, pubmed-meshheading:15487890-Humans, pubmed-meshheading:15487890-Iron, pubmed-meshheading:15487890-Macrophages, pubmed-meshheading:15487890-Oxidation-Reduction, pubmed-meshheading:15487890-Oxidative Stress, pubmed-meshheading:15487890-Particulate Matter, pubmed-meshheading:15487890-Physicochemical Phenomena, pubmed-meshheading:15487890-Porosity
pubmed:year
2004
pubmed:articleTitle
Macrophage-mediated endothelial inflammatory responses to airborne particulates: impact of particulate physicochemical properties.
pubmed:affiliation
Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.