Source:http://linkedlifedata.com/resource/pubmed/id/15486935
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2004-10-25
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| pubmed:abstractText |
Differentiation of human embryonic stem cells (hESCs) can be instigated through the formation of embryo-like aggregates in suspension, termed human embryoid bodies (hEBs). Controlling cell aggregation and agglomeration during hEBs formation has a profound effect on the extent of cell proliferation and differentiation. In a previous work, we showed that control over hEBs formation and differentiation can be achieved via cultivation of hESC suspensions in a rotating bioreactor system. We now report that hEBs can be generated directly from hESC suspensions within three-dimensional (3D) porous alginate scaffolds. The confining environments of the alginate scaffold pores enabled efficient formation of hEBs with a relatively high degree of cell proliferation and differentiation; encouraged round, small-sized hEBs; and induced vasculogenesis in the forming hEBs to a greater extent than in static or rotating cultures. We therefore conclude that differentiation of hEBs can be induced and directed by physical constraints in addition to chemical cues.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alginates,
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Glucuronic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Hexuronic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial,
http://linkedlifedata.com/resource/pubmed/chemical/alginic acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0006-3592
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2004 Wiley Periodicals, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
5
|
| pubmed:volume |
88
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
313-20
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15486935-Alginates,
pubmed-meshheading:15486935-Bioartificial Organs,
pubmed-meshheading:15486935-Biocompatible Materials,
pubmed-meshheading:15486935-Cell Culture Techniques,
pubmed-meshheading:15486935-Cell Differentiation,
pubmed-meshheading:15486935-Cell Line,
pubmed-meshheading:15486935-Cell Proliferation,
pubmed-meshheading:15486935-Cell Size,
pubmed-meshheading:15486935-Cell Survival,
pubmed-meshheading:15486935-Embryo, Mammalian,
pubmed-meshheading:15486935-Embryonic Development,
pubmed-meshheading:15486935-Glucuronic Acid,
pubmed-meshheading:15486935-Hexuronic Acids,
pubmed-meshheading:15486935-Humans,
pubmed-meshheading:15486935-Materials Testing,
pubmed-meshheading:15486935-Membranes, Artificial,
pubmed-meshheading:15486935-Neovascularization, Physiologic,
pubmed-meshheading:15486935-Porosity,
pubmed-meshheading:15486935-Stem Cells,
pubmed-meshheading:15486935-Tissue Engineering
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Three-dimensional porous alginate scaffolds provide a conducive environment for generation of well-vascularized embryoid bodies from human embryonic stem cells.
|
| pubmed:affiliation |
Biotechnology Interdisciplinary Unit, Technion-Israel Institute of Technology, Haifa, Israel.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|