Linked Life Data

15485810

Source:http://linkedlifedata.com/resource/pubmed/id/15485810

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
53
pubmed:dateCreated
2004-12-23
pubmed:abstractText
The phage shock protein operon (pspABCDE) of Escherichia coli is strongly up-regulated in response to overexpression of the filamentous phage secretin protein IV (pIV) and by many other stress conditions including defects in protein export. PspA has an established role in maintenance of the proton-motive force of the cell under stress conditions. Here we present evidence for a new member of the phage shock response in E. coli. Using transcriptional profiling, we show that the synthesis of pIV in E. coli leads to a highly restricted response limited to the up-regulation of the psp operon genes and yjbO. The psp operon and yjbO are also up-regulated in response to pIV in Salmonella enterica serovar Typhimurium. yjbO is a highly conserved gene found exclusively in bacteria that contain a psp operon but is physically unlinked to the psp operon. yjbO encodes a putative inner membrane protein that is co-controlled with the psp operon genes and is predicted to be an effector of the psp response in E. coli. We present evidence that yjbO expression is driven by sigma(54)-RNA polymerase, activated by PspF and integration host factor, and negatively regulated by PspA. PspF specifically regulates only members of the PspF regulon: pspABCDE and yjbO. We found that increased expression of YjbO results in decreased motility of bacteria. Because yjbO is co-conserved and co-regulated with the psp operon and is a member of the phage shock protein F regulon, we propose that yjbO be renamed pspG.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55707-14
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:15485810-Bacterial Proteins, pubmed-meshheading:15485810-Base Sequence, pubmed-meshheading:15485810-Blotting, Western, pubmed-meshheading:15485810-Cell Membrane, pubmed-meshheading:15485810-Cell Movement, pubmed-meshheading:15485810-DNA, Complementary, pubmed-meshheading:15485810-Dose-Response Relationship, Drug, pubmed-meshheading:15485810-Escherichia coli, pubmed-meshheading:15485810-Escherichia coli Proteins, pubmed-meshheading:15485810-Gene Expression Regulation, pubmed-meshheading:15485810-Genetic Vectors, pubmed-meshheading:15485810-Genome, pubmed-meshheading:15485810-Genome, Bacterial, pubmed-meshheading:15485810-Heat-Shock Proteins, pubmed-meshheading:15485810-Membrane Proteins, pubmed-meshheading:15485810-Molecular Sequence Data, pubmed-meshheading:15485810-Mutation, pubmed-meshheading:15485810-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:15485810-Plasmids, pubmed-meshheading:15485810-Protons, pubmed-meshheading:15485810-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15485810-Salmonella enterica, pubmed-meshheading:15485810-Trans-Activators, pubmed-meshheading:15485810-Transcription, Genetic, pubmed-meshheading:15485810-Up-Regulation, pubmed-meshheading:15485810-beta-Galactosidase
pubmed:year
2004
pubmed:articleTitle
Identification of a new member of the phage shock protein response in Escherichia coli, the phage shock protein G (PspG).
pubmed:affiliation
Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't