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pubmed-article:15483381pubmed:abstractTextCarbohydrate-carbohydrate recognition is emerging today as an important type of interaction in cell adhesion. One Ca(2+)mediated homotypic interaction between two Lewis( X ) determinants (Le( X )) has been proposed to drive cell adhesion in murine embryogenesis. Here, the adhesion energies of lipid vesicles functionalized with glycolipids bearing monomeric or dimeric Le( X ) determinants were measured in NaCl or CaCl(2) media with the micropipette aspiration technique. These experiments on Le( X ) with an environment akin to that provided by biological membrane confirmed the existence of this specific calcium dependent interaction of monomeric Le( X ). In contrast, dimeric Le( X ) produced a repulsive contribution. By using a simple model involving the various contributions to the adhesion free energy, specific and non specific interactions could be separated and quantified. The involvement of calcium ions has been discussed in the monomeric and dimeric Le( X ) lipids.lld:pubmed
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pubmed-article:15483381pubmed:authorpubmed-author:SinaÿPierrePlld:pubmed
pubmed-article:15483381pubmed:authorpubmed-author:ZhangYongminYlld:pubmed
pubmed-article:15483381pubmed:authorpubmed-author:PincetFrédéri...lld:pubmed
pubmed-article:15483381pubmed:authorpubmed-author:PerezEricElld:pubmed
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pubmed-article:15483381pubmed:authorpubmed-author:GourierChrist...lld:pubmed
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pubmed-article:15483381pubmed:pagination165-74lld:pubmed
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pubmed-article:15483381pubmed:year2004lld:pubmed
pubmed-article:15483381pubmed:articleTitleSpecific and non specific interactions involving Le( X ) determinant quantified by lipid vesicle micromanipulation.lld:pubmed
pubmed-article:15483381pubmed:affiliationLaboratoire de Physique Statistique de l'Ecole Normale Supérieure, UMR 8550 associée au CNRS et aux universités Paris 6 et Paris 7, France.lld:pubmed
pubmed-article:15483381pubmed:publicationTypeJournal Articlelld:pubmed
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