Linked Life Data

15481963

Source:http://linkedlifedata.com/resource/pubmed/id/15481963

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2004-10-14
pubmed:abstractText
Biochip surfaces for immobilization of DNA and proteins require reactive polymers with high immobilization capacity and low nonspecific binding. Most reactive surfaces consist of matrixes that provide epoxy, aldehyde, or amino functions for biomolecule binding. The most widely used oligonucleotide modification is a C6-amino link. The high reactivity of isothiocyanate groups (NCS) toward amines was therefore the motivation to employ photogenerated NCS groups as binding sites for NH(2)-terminated oligonucleotides. Photosensitive poly(styrene-co-4-vinylbenzyl thiocyanate) (PST-co-VBT) was synthesized and applied as novel material for DNA and protein immobilization. The immobilization capacity of PST-co-VBT was a function of UV energy density used for photoactivation and was approximately 80% at 450 mJ cm(-)(2) (lambda(ex) = 254 nm). This surface was superior to tested commercial chip surfaces in signal-to-noise-ratio and reproducibility. Print buffer and spacer length were optimized for maximum fluorescence signal with DNA and proteins. UV exposure conditions and oligonucleotide modification were correlated, showing that this photochemical approach can be successfully applied for surface patterning of biochips.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6130-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Photoactivatable copolymers of vinylbenzyl thiocyanate as immobilization matrix for biochips.
pubmed:affiliation
Biotechnology Unit and Environmental Research Unit, Division of Environmental and Life Sciences, ARC Seibersdorf Research GmbH, A-2444 Seibersdorf, Austria. claudia.preininger@arcs.ac.at
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't