| pubmed-article:15480750 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C1706048 | lld:lifeskim |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C0001430 | lld:lifeskim |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C2936239 | lld:lifeskim |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C0003075 | lld:lifeskim |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C1335836 | lld:lifeskim |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C1621574 | lld:lifeskim |
| pubmed-article:15480750 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:15480750 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:15480750 | pubmed:dateCreated | 2005-2-2 | lld:pubmed |
| pubmed-article:15480750 | pubmed:abstractText | Electroneutral NaCl absorption in the intestine is mediated by parallel Na+/H+ and Cl-/HCO3- exchange. Mutations in the down-regulated in adenoma (DRA) gene cause congenital chloride diarrhoea but the transport characteristics of human DRA have not been studied in a heterologous human expression system. A N-terminal enhanced green fluorescence protein (EGFP)-tagged human DRA construct was therefore expressed stably in HEK293 cells. Cl-/HCO3- exchange was assessed by measuring intracellular pH and intracellular Cl- using fluorescent dyes. Expression of DRA resulted in the appearance of EGFP fluorescence and DRA immunoreactivity consistent with a location in the plasma membrane and possibly structures below the plasma membrane. DRA mediated electroneutral Cl-/HCO3- exchange but OH- was not transported and SO4(2-)/HCO3- exchange was minimal. In the presence of 5% CO2/HCO3- the apparent affinity of DRA for Cl- in transfected HEK cells was 23-36 mM, which is lower than that reported for rabbit ileal brush border membrane vesicles and for oocytes injected with human DRA. DRA was inhibited by 4 mM DIDS (45+/-11%), by 50 microM tenidap (71+/-8%) and by 100 microM glibenclamide (59+/-22% inhibition of HCO3- transport and 79+/-3% inhibition of Cl- transport). The effects of DIDS and tenidap were not additive to those of glibenclamide. | lld:pubmed |
| pubmed-article:15480750 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15480750 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15480750 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15480750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15480750 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15480750 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15480750 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:15480750 | pubmed:issn | 0031-6768 | lld:pubmed |
| pubmed-article:15480750 | pubmed:author | pubmed-author:LamprechtGeor... | lld:pubmed |
| pubmed-article:15480750 | pubmed:author | pubmed-author:GregorMichael... | lld:pubmed |
| pubmed-article:15480750 | pubmed:author | pubmed-author:BaischSusanna... | lld:pubmed |
| pubmed-article:15480750 | pubmed:author | pubmed-author:SchoenleberEl... | lld:pubmed |
| pubmed-article:15480750 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15480750 | pubmed:volume | 449 | lld:pubmed |
| pubmed-article:15480750 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15480750 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15480750 | pubmed:pagination | 479-90 | lld:pubmed |
| pubmed-article:15480750 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:15480750 | pubmed:meshHeading | pubmed-meshheading:15480750... | lld:pubmed |
| pubmed-article:15480750 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15480750 | pubmed:articleTitle | Transport properties of the human intestinal anion exchanger DRA (down-regulated in adenoma) in transfected HEK293 cells. | lld:pubmed |
| pubmed-article:15480750 | pubmed:affiliation | 1st Department of Medicine, University of Tübingen, 72076, Tübingen, Germany. hans-georg.lamprecht@uni-tuebingen.de | lld:pubmed |
| pubmed-article:15480750 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15480750 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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