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rdf:type |
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lifeskim:mentions |
umls-concept:C0015980,
umls-concept:C0028326,
umls-concept:C0029348,
umls-concept:C0033684,
umls-concept:C0086860,
umls-concept:C1514562,
umls-concept:C1706044,
umls-concept:C1707271,
umls-concept:C1879547,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
21
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pubmed:dateCreated |
2004-10-13
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pubmed:abstractText |
The NS1 proteins of influenza A and B viruses (A/NS1 and B/NS1 proteins) have only approximately 20% amino acid sequence identity. Nevertheless, these proteins show several functional similarities, such as their ability to bind to the same RNA targets and to inhibit the activation of protein kinase R in vitro. A critical function of the A/NS1 protein is the inhibition of synthesis of alpha/beta interferon (IFN-alpha/beta) during viral infection. Recently, it was also found that the B/NS1 protein inhibits IFN-alpha/beta synthesis in virus-infected cells. We have now found that the expression of the B/NS1 protein complements the growth of an influenza A virus with A/NS1 deleted. Expression of the full-length B/NS1 protein (281 amino acids), as well as either its N-terminal RNA-binding domain (amino acids 1 to 93) or C-terminal domain (amino acids 94 to 281), in the absence of any other influenza B virus proteins resulted in the inhibition of IRF-3 nuclear translocation and IFN-beta promoter activation. A mutational analysis of the truncated B/NS1(1-93) protein showed that RNA-binding activity correlated with IFN-beta promoter inhibition. In addition, a recombinant influenza B virus with NS1 deleted induces higher levels of IRF-3 activation, as determined by its nuclear translocation, and of IFN-alpha/beta synthesis than wild-type influenza B virus. Our results support the hypothesis that the NS1 protein of influenza B virus plays an important role in antagonizing the IRF-3- and IFN-induced antiviral host responses to virus infection.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-10024172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-10205180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-10725408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-10920397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-10933707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-11027311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-11090154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-11157743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-11421366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-12114540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-12368362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-12438621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-12502864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-12504570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-12517228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-12692549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-12702806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-12915566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-14645582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-14747551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-14967035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-1660837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-3811235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-7958859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-8139028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-8207822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-8313914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-8806538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-9360601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-9360602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-9463386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-9541017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-9566918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-9651582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-9660935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-9865487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15479798-9878611
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11574-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15479798-Active Transport, Cell Nucleus,
pubmed-meshheading:15479798-Animals,
pubmed-meshheading:15479798-Cell Line,
pubmed-meshheading:15479798-DNA-Binding Proteins,
pubmed-meshheading:15479798-Gene Expression Regulation,
pubmed-meshheading:15479798-Humans,
pubmed-meshheading:15479798-Influenza B virus,
pubmed-meshheading:15479798-Interferon Regulatory Factor-3,
pubmed-meshheading:15479798-Interferon-beta,
pubmed-meshheading:15479798-Promoter Regions, Genetic,
pubmed-meshheading:15479798-Protein Transport,
pubmed-meshheading:15479798-RNA,
pubmed-meshheading:15479798-Transcription Factors,
pubmed-meshheading:15479798-Viral Nonstructural Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
The N- and C-terminal domains of the NS1 protein of influenza B virus can independently inhibit IRF-3 and beta interferon promoter activation.
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pubmed:affiliation |
Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L. Levy Pl., New York, NY 10029, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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