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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2004-10-13
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pubmed:abstractText |
Heterotrimeric G proteins are crucial for asymmetric cell division, but the mechanisms of signal activation remain poorly understood. Here, we establish that the evolutionarily conserved protein RIC-8 is required for proper asymmetric division of one-cell stage C. elegans embryos. Spindle severing experiments demonstrate that RIC-8 is required for generation of substantial pulling forces on astral microtubules. RIC-8 physically interacts with GOA-1 and GPA-16, two Galpha subunits that act in a partially redundant manner in one-cell stage embryos. RIC-8 preferentially binds to GDP bound GOA-1 and is a guanine nucleotide exchange factor (GEF) for GOA-1. Our analysis suggests that RIC-8 acts before the GoLoco protein GPR-1/2 in the sequence of events leading to Galpha activation. Furthermore, coimmunoprecipitation and in vivo epistasis demonstrate that inactivation of the Gbeta subunit GPB-1 alleviates the need for RIC-8 in one-cell stage embryos. Our findings suggest a mechanism in which RIC-8 favors generation of Galpha free from Gbetagamma and enables GPR-1/2 to mediate asymmetric cell division.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/G protein regulator 1, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/G protein regulator 2, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Dissociation...,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Exchange Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RIC-8 protein, C elegans
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
119
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
219-30
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15479639-Animals,
pubmed-meshheading:15479639-Caenorhabditis elegans,
pubmed-meshheading:15479639-Caenorhabditis elegans Proteins,
pubmed-meshheading:15479639-Cell Division,
pubmed-meshheading:15479639-Embryo, Nonmammalian,
pubmed-meshheading:15479639-Enzyme Activation,
pubmed-meshheading:15479639-Epistasis, Genetic,
pubmed-meshheading:15479639-GTP-Binding Protein alpha Subunits,
pubmed-meshheading:15479639-Guanine Nucleotide Dissociation Inhibitors,
pubmed-meshheading:15479639-Guanine Nucleotide Exchange Factors,
pubmed-meshheading:15479639-Microtubules,
pubmed-meshheading:15479639-Mitotic Spindle Apparatus,
pubmed-meshheading:15479639-Nuclear Proteins,
pubmed-meshheading:15479639-Protein Binding,
pubmed-meshheading:15479639-RNA Interference,
pubmed-meshheading:15479639-Stress, Mechanical,
pubmed-meshheading:15479639-Two-Hybrid System Techniques
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pubmed:year |
2004
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pubmed:articleTitle |
RIC-8 is required for GPR-1/2-dependent Galpha function during asymmetric division of C. elegans embryos.
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pubmed:affiliation |
Swiss Institute for Experimental Cancer Research (ISREC), 1066 Epalinges/Lausanne, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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