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rdf:type |
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lifeskim:mentions |
umls-concept:C0017278,
umls-concept:C0019682,
umls-concept:C0019699,
umls-concept:C0039195,
umls-concept:C0056912,
umls-concept:C0061830,
umls-concept:C0205263,
umls-concept:C0332307,
umls-concept:C0871261,
umls-concept:C1422036,
umls-concept:C1514562,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
5
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pubmed:dateCreated |
2004-10-13
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pubmed:abstractText |
In the present study we investigated the immunomodulatory effects of two adjuvants, liposomal lipid A [L(LA)] and CpG-containing oligodeoxynucleotides (CpG ODN), to the HIV-1 ogp140 envelope protein. Administration of each of these adjuvants separately with unencapsulated ogp140 resulted in low antibody titres. Encapsulation of ogp140 in liposomes containing lipid A resulted in a sixfold increase in anti-ogp140 antibodies. The antibody titres were further enhanced threefold by the addition of CpG ODN. Priming and boosting BALB/c mice with unencapsulated ogp140 with L(LA) or encapsulation in liposomes containing lipid A induced a mixed Th1/Th2 type of immune response. In contrast, immunization with L(ogp140 + LA) plus CpG ODN switched the immune response to a Th-1 response with elevated anti-ogp140 IgG2a antibodies and IFN-gamma levels. Both adjuvants induced excellent ogp140-specific proliferative and CTL responses. Therefore, for the induction of high titre antibodies, but not for cellular responses, the antigen and lipid A have to be present in the same liposomes. These results can have significant implications in directing the Th1 or Th2 differentiation of antigen-specific immune responses in the context of vaccine development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, env,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid A,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/env Gene Products, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/gp140 envelope protein, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0818-9641
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2004 Australasian Society for Immunology Inc.
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
523-30
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15479438-Adjuvants, Immunologic,
pubmed-meshheading:15479438-Animals,
pubmed-meshheading:15479438-Antibody Formation,
pubmed-meshheading:15479438-CpG Islands,
pubmed-meshheading:15479438-Dimerization,
pubmed-meshheading:15479438-Female,
pubmed-meshheading:15479438-Gene Products, env,
pubmed-meshheading:15479438-HIV-1,
pubmed-meshheading:15479438-Lipid A,
pubmed-meshheading:15479438-Liposomes,
pubmed-meshheading:15479438-Mice,
pubmed-meshheading:15479438-Mice, Inbred BALB C,
pubmed-meshheading:15479438-Oligodeoxyribonucleotides,
pubmed-meshheading:15479438-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:15479438-Th1 Cells,
pubmed-meshheading:15479438-Th2 Cells,
pubmed-meshheading:15479438-env Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2004
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pubmed:articleTitle |
Immunostimulatory CpG motifs induce CTL responses to HIV type I oligomeric gp140 envelope protein.
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pubmed:affiliation |
Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Silver Spring, MD 20910-7500, USA. Mangala.Rao@na.amedd.army.mil
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pubmed:publicationType |
Journal Article
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