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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2004-10-12
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pubmed:abstractText |
Apurinic/apyrimidinic (AP) sites are alkali labile lesions that, when encountered during DNA replication, can block polymerases or potentially result in mutagenic events. Owing to the instability of 2-deoxyribose lesions (AP), a chemically stable tetrahydrofuran analog (F) is often used as a model of abasic sites. A comparison of the two lesions in Saccharomyces cerevisiae revealed that the model lesion and 2-deoxyribose have distinct in vivo effects. Comprehensive comparative analyses of F and AP have not been carried out in Escherichia coli. We conducted a side-by-side investigation of F and AP in E.coli to compare their biological effects and interactions with SOS polymerases. Both lesions were examined in SOS-induced and uninduced cells. Our studies reveal that in uninduced E.coli the effects of individual polymerases in the replication of plasmids containing F or AP are distinct. However, when cells are SOS-induced, the biological effects of F and AP are similar.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-10508678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-10754553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-10801133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-11080171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-11316789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-11790114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-11891323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-12060704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-12466536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-12676985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-14599744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-15023344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-15157106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-2186377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-2440861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-3293048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-3741336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-6287922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-6354260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-6453298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-7643399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-7908652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-8798733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-9016601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15477395-9153253
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5480-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15477395-DNA, Bacterial,
pubmed-meshheading:15477395-DNA Damage,
pubmed-meshheading:15477395-DNA Replication,
pubmed-meshheading:15477395-DNA-Directed DNA Polymerase,
pubmed-meshheading:15477395-Deoxyribose,
pubmed-meshheading:15477395-Escherichia coli,
pubmed-meshheading:15477395-Furans,
pubmed-meshheading:15477395-Mutation,
pubmed-meshheading:15477395-Nucleotides,
pubmed-meshheading:15477395-Plasmids,
pubmed-meshheading:15477395-SOS Response (Genetics),
pubmed-meshheading:15477395-Saccharomyces cerevisiae
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pubmed:year |
2004
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pubmed:articleTitle |
A comprehensive comparison of DNA replication past 2-deoxyribose and its tetrahydrofuran analog in Escherichia coli.
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pubmed:affiliation |
Department of Chemistry, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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